UBE2L3 Antibody, HRP conjugated
Shipped with Ice Packs 
In Stock 
Description
2.1. Detection of UBE2L3 in Cellular and Tissue Samples
The HRP-conjugated antibody is used to study UBE2L3’s role in:
-
Protein Degradation: Mediates ubiquitination of short-lived or abnormal proteins via interactions with E3 ligases (e.g., E6-AP, c-Cbl) .
-
NF-κB Signaling: Critical in TNFα-induced linear ubiquitination of NEMO, enabling IKK complex activation and proinflammatory cytokine production .
-
Mitophagy: Essential for Parkin-dependent mitophagy in neuronal cells .
Positive Samples Detected by UBE2L3 Antibody
| Sample Type | Species | Applications | Source |
|---|---|---|---|
| HeLa cells | Human | WB, IP, IHC | |
| Mouse kidney/testis | Mouse | WB, IP, IHC | |
| Human lung cancer | Human | IHC (with antigen retrieval) | |
| Platelets | Human | WB (thrombosis studies) |
3.1. Role in Inflammatory Pathways
-
TNFα Signaling: UBE2L3 knockdown reduces TNFα-induced linear ubiquitination of NEMO, impairing NF-κB activation and cytokine transcription (e.g., IL-8, IL-6) .
-
Autoimmune Diseases: Genetic variants in UBE2L3 are linked to systemic lupus erythematosus (SLE), rheumatoid arthritis, and Crohn’s disease, correlating with enhanced UBE2L3 expression .
3.2. Mechanistic Studies
-
LUBAC Complex Interaction: UBE2L3 associates with HOIL-1L in the linear ubiquitin chain assembly complex (LUBAC), enabling linear polyubiquitination of NEMO in vivo .
-
Distinctive E2 Activity: Unlike UBE2D3, UBE2L3 activates NF-κB reporters and interacts with LUBAC components, establishing its specificity in linear ubiquitination .
4.1. Biomarker Potential
UBE2L3’s elevated expression in autoimmune diseases suggests its utility as a diagnostic marker or therapeutic target .
4.2. Limitations and Challenges
Product Specs
Buffer
**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Typically, we can ship your order within 1-3 business days of receipt. Delivery times may vary depending on the method of purchase and location. For specific delivery details, please consult your local distributor.
Synonyms
E2 F1 antibody; L UBC antibody; L-UBC antibody; UB2L3_HUMAN antibody; UBCE7 antibody; UbcH7 antibody; UbcM4 antibody; Ube2l3 antibody; Ubiquitin carrier protein L3 antibody; Ubiquitin conjugating enzyme E2 L3 antibody; Ubiquitin protein ligase L3 antibody; Ubiquitin-conjugating enzyme E2 L3 antibody; Ubiquitin-conjugating enzyme E2-F1 antibody; Ubiquitin-protein ligase L3 antibody
Target Names
Uniprot No.
Target Background
Function
UBE2L3, also known as ubiquitin conjugating enzyme E2L3, is a ubiquitin-conjugating enzyme that specifically interacts with HECT-type and RBR family E3 ubiquitin-protein ligases. It lacks the intrinsic E3-independent reactivity with lysine that is typical of most RING-containing E3 ubiquitin-protein ligases, but it demonstrates activity with RBR family E3 enzymes, such as PRKN and ARIH1, which function like RING-HECT hybrids. UBE2L3 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, it catalyzes 'Lys-11'-linked polyubiquitination. UBE2L3 plays a role in the selective degradation of short-lived and abnormal proteins. It is downregulated during the S-phase and is involved in cell cycle progression. Additionally, it regulates the transcriptional activity of nuclear hormone receptors. UBE2L3 may also be involved in myelopoiesis.
Gene References Into Functions
- Crystal structure of HHARI and UbcH7 reveals UbcH7 approximately Ub binding RING1 domain of auto-inhibited HHARI. PMID: 28552575
- Data suggest ordered binding of UBCH7-ubiquitin to E6AP Site 1 (for E6AP-Cys820/ubiquitin thioester formation) and E6AP Site 2 (for subsequent chain elongation); proximal indexation accounts for radially symmetric structure of E6AP, requirement for oligomerization in polyubiquitin chain formation, and mechanistic rationale for Cys820-ubiquitin thioester as platform in chain assembly. (E6AP = ubiquitin-protein ligase E6AP) PMID: 28924046
- The TCGGC haplotype is associated with an increased risk of Hashimoto Thyroiditis(HT) and UBE2L3 gene is likely to be a susceptibility factor to HT in a Chinese Han population. PMID: 27094594
- together with UBE2N and UBE2D2, synergistically contribute to Parkin-mediated mitophagy PMID: 24906799
- The MAP1B-LC1 could interact with the ubiquitin-conjugating E2 enzyme UBE2L3 and that the ubiquitination/degradation mechanism triggered by MAP1B-LC1 could be prevented by inhibiting the ubiquitin-proteasome proteolytic pathway. PMID: 25483588
- An interaction between LC1 and the ubiquitin-conjugating enzyme UBE2L3. PMID: 24566975
- UbcH7 has a role in regulating 53BP1 stability and DSB repair PMID: 25422456
- UBE2L3 polymorphism amplifies NF-kappaB activation and promotes plasma cell development, linking linear ubiquitination to multiple autoimmune diseases. PMID: 25640675
- Report role of UBE2L3 genetic variants in confering risk of systemic lupus erythematosus in Chinese population. PMID: 24091983
- Importance of HLA-C and UBE2L3 in the clearance of HBV infection in addition to HLA-DP and HLA-DQ. PMID: 24162738
- The results suggest that variants carried on the SLE-associated UBE2L3 risk haplotype influence autoimmunity by modulating UBCH7 expression. PMID: 22476155
- UBE2L3 was found to be a shared susceptibility gene for siffuse cutaneous systemic sclerosis in a Japanese population. PMID: 22294623
- The interaction between UBE2L3 genotype and autoantibodies upon serum IFN-alpha suggests a biological role for this locus in patients with systemic lupus erythematosus. PMID: 22045845
- Crystal structures of two bacterial E3s, Salmonella SopA and Escherichia coli NleL, both in complex with human E2 UbcH7, are reported. PMID: 22308380
- UBCH7 exhibits activity with the RING-in-between-RING (RBR) family of E3s that includes parkin (also known as PARK2) and human homologue of ariadne (HHARI; also known as ARIH1) PMID: 21532592
- Genetic variations of UBE2L3 and BCL3 are potential new risk genes for Crohn's disease. PMID: 20601676
- UbcH7 charged with ubiquitin has an affinity for E6-associated protein (E6AP) similar to that of uncharged UbcH7. PMID: 20039703
- UBE2L3, the c-myc-regulated gene is involved in genotype-C-HBV-related HCC, suggesting that c-myc is related to the hepatocarcinogenic activity of genotype-C HBV. PMID: 16703398
- identification of the amino acid residues responsible for the HECT-E2 interaction, and for the dynamical properties of the ubiquitin transfer process, may be of relevant interest for pharmacological and therapeutical purposes PMID: 18805400
- These data suggest a role for UbcH7 targets in the completion of cell cycle S phase and entry into G2. PMID: 18946090
Database Links
Protein Families
Ubiquitin-conjugating enzyme family
Subcellular Location
Nucleus. Cytoplasm.
Tissue Specificity
Ubiquitous, with highest expression in testis.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
