UBE4B Antibody
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Description
Definition and Target Specificity
UBE4B antibodies are immunoreagents designed to detect the UBE4B protein, a ubiquitin ligase involved in polyubiquitination and degradation of substrates like p53, Tau, and KLHL22. These antibodies are validated for use in techniques such as Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF/ICC) .
Research Applications and Findings
UBE4B antibodies have been instrumental in elucidating the protein’s role in cancer, neurodegeneration, and cellular signaling.
Table 1: Key Research Findings Using UBE4B Antibodies
Technical Considerations
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Validation: Antibodies are tested across cell lines (e.g., HEK-293T, HeLa) and tissues (e.g., human heart, mouse testis) .
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Knockdown/Knockout Validation: Used in siRNA and CRISPR models to confirm UBE4B’s role in substrate degradation (e.g., p53, KLHL22) .
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Cross-Reactivity: No reported cross-reactivity with UBE4A or other E3 ligases .
Pathological and Therapeutic Implications
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Cancer: UBE4B promotes tumorigenesis by degrading p53 and enhancing MDM2 activity . High UBE4B expression in renal and lung cancers predicts poor survival .
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Neurodegeneration: UBE4B deficiency exacerbates Aβ42 accumulation in Alzheimer’s models and impairs Tau clearance .
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Therapeutic Target: Small-molecule inhibitors of UBE4B are under exploration for cancers and mTOR-related disorders .
Limitations and Future Directions
Product Specs
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. Stored at -20°C. Avoid freeze-thaw cycles.
Lead Time
Typically, we can ship products within 1-3 business days after receiving your order. Delivery times may vary depending on the purchase method or location. Please consult your local distributor for specific delivery timeframes.
Synonyms
4930551I19Rik antibody; 4933406G05Rik antibody; AU014668 antibody; D4Bwg0973e antibody; E4 antibody; HDNB1 antibody; Homozygously deleted in neuroblastoma 1 antibody; KIAA0684 antibody; mKIAA0684 antibody; RP23 173D8.1 antibody; Ube4b antibody; UBE4B_HUMAN antibody; Ubiquitin conjugation factor E4 B antibody; Ubiquitin fusion degradation protein 2 antibody; UBOX3 antibody; UFD2 antibody; UFD2a antibody; Ufd2p antibody
Target Names
Uniprot No.
Target Background
Function
UBE4B is a ubiquitin-protein ligase that is likely involved in ubiquitination as an E3 ligase, collaborating with specific E1 and E2 ligases. It may also function as an E4 ligase, facilitating the assembly of polyubiquitin chains on substrates already ubiquitinated by another E3 ubiquitin ligase. UBE4B, in conjunction with STUB1 and VCP/p97, might play a role in regulating myosin assembly in striated muscles by targeting the myosin chaperone UNC45B for proteasomal degradation.
Gene References Into Functions
- miR-1301 acts as a tumor suppressor by inhibiting tumor cell migration and invasion in various human cancer cells. This suppression is achieved through the regulation of the UBE4B-p53 pathway. PMID: 28483517
- UBE4B facilitates the degradation of endogenous phospho-p53(S15) and phospho-p53(S392) in response to gamma irradiation. This suggests a crucial role for UBE4B in regulating phosphorylated p53 following DNA damage. PMID: 26673821
- UBE4B overexpression promotes the oncogenic properties of hepatocellular carcinoma (HCC) and is associated with an unfavorable prognosis in HCC patients. PMID: 25557723
- UBE4B regulates p53 in breast cancer. PMID: 24587254
- A proposed model suggests that the combined actions of UBE4B, ESCRT-0, and the deubiquitinating enzyme USP8 enable the endosomal sorting and lysosomal degradation of the epidermal growth factor receptor (EGFR). PMID: 24344129
- UBE4B-mediated growth factor receptor trafficking might contribute to the poor prognosis of patients with neuroblastoma tumors exhibiting 1p36 deletions. PMID: 22990745
- The regulated expression of different UFD2a isoforms likely leads to distinct functions that are essential for myogenesis. PMID: 22174917
- Somatic mutations of HERC2, HERC3, TRIP12, UBE2Q1, and UBE4B genes have been identified in gastric carcinoma and colorectal carcinomas with microsatellite instability. PMID: 22124266
- Evidence suggests that amplification and overexpression of UBE4B represent previously unknown molecular mechanisms of p53 inactivation in brain tumors. PMID: 21317885
- The structures of the E4B U box in its free form and bound to UbcH5c and Ubc4 E2s have been determined. These findings indicate that the E4B U box is a monomer stabilized by a network of hydrogen bonds. The results suggest an allosteric regulation of UbcH5c and Ubc4 by the E4B U box. PMID: 20696396
- A splice site mutation was found in UBE4b. UBE4B expression was reduced in high-stage/poor-outcome tumors. This suggests that UBE4B is a strong candidate for a tumor suppressor gene in neuroblastoma. PMID: 12700669
- E4B serves as a ubiquitin ligase for FEZ1, thereby regulating its function but not its degradation. PMID: 15466860
- A novel domain, termed the MPAC domain (Mitotically Phosphorylated, Apoptotically Cleaved), has been identified at the N-terminus of Ufd2a. This domain is regulated by cleavage during cell death and phosphorylation during mitosis. PMID: 16870146
- Recent research provides insights into the regulation of DeltaNp63alpha protein levels in response to cisplatin, suggesting that UFD2a might play a significant role in the regulation of cisplatin-mediated cell death involving p63. PMID: 18418053
Database Links
Protein Families
Ubiquitin conjugation factor E4 family
Subcellular Location
Cytoplasm. Nucleus.
Tissue Specificity
Expressed in differentiated myotubes (at protein level). Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
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