UBN2 Antibody
Description
UBN2 Antibody Characterization
UBN2 antibodies are primarily used in research applications such as Western Blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Key validated antibodies include:
Validation Standards:
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Antibodies are tested using knockout (KO) cell lines to confirm specificity .
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Prestige Antibodies® (e.g., HPA020124) are validated across 44 normal and 20 cancer tissues .
Cancer Relevance
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Colorectal Cancer (CRC): UBN2 is a direct target of tumor-suppressive miR-802. Overexpression of miR-802 inhibits CRC cell proliferation and invasion by downregulating UBN2 .
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Prostate Cancer: UBN2 is part of the ADAM9/UBN2/AKR1C3 axis, promoting resistance to androgen-deprivation therapy .
Top Antibodies and Performance
A 2023 study evaluated ten commercial Ubiquilin-2 (UBQLN2) antibodies, emphasizing the need for KO-validated reagents . While this study focused on UBQLN2, the methodology informs UBN2 antibody validation:
| Application | High-Performing Antibodies Identified | Key Metrics |
|---|---|---|
| Western Blot | 6/10 antibodies | Specificity confirmed via KO cell lines |
| Immunoprecipitation | 4/10 antibodies | Effective target enrichment |
| Immunofluorescence | 5/10 antibodies | Subcellular localization accuracy |
Recommended Workflow
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Western Blot: Use HAP1 WT and KO cells for specificity checks .
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Functional Studies: Pair UBN2 antibodies with siRNA knockdown to assess phenotypic effects (e.g., senescence or transcription recovery) .
Challenges and Best Practices
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Cross-Reactivity: Antibodies targeting UBN2 may cross-react with UBN1 due to structural similarities .
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Validation: Always use orthogonal methods (e.g., CRISPR-KO controls) to confirm antibody specificity .
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Storage: Maintain antibodies at −20°C in glycerol-based buffers to preserve activity .
Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
