Customize ufc-1 Antibody

Product Specs

Buffer

**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

ufc-1 antibody; C40H1.6Ubiquitin-fold modifier-conjugating enzyme 1 antibody; Ufm1-conjugating enzyme 1 antibody

Target Names

ufc-1

Uniprot No.

Q03598

Target Background

Function

UFC-1 is an E2-like enzyme that forms an intermediate with UFM1. This intermediate is formed via a thioester linkage.

Database Links

KEGG: cel:CELE_C40H1.6

STRING: 6239.C40H1.6.1

UniGene: Cel.10720

Protein Families

Ubiquitin-conjugating enzyme family, UFC1 subfamily

Tissue Specificity

Expressed in the intestine.

Q&A

Basic Research Questions

What validation methods ensure UFC1 antibody specificity in experimental models?

Perform Western blotting using UFC1-knockout cell lysates as negative controls
Conduct peptide blocking assays with antigenic peptides to confirm binding specificity
Validate through immunoprecipitation-mass spectrometry to identify interacting partners

How to optimize UFC1 antibody protocols for diverse applications?

Application	Key Parameters	Validation Example
Western Blot	5% BSA blocking, 1:500 dilution	Human HEK293T lysates
IHC-P	Antigen retrieval (pH 6.0 citrate buffer)	NSCLC patient tissues
IF	0.1% Triton X-100 permeabilization	Cervical cancer cell lines

What controls are essential when studying UFC1 in ufmylation pathways?

Include UBA5/UFL1 knockout models to confirm enzymatic cascade dependency
Use ATP-depleted systems to verify energy requirement for UFM1 conjugation
Validate with recombinant UFC1 protein in in vitro ufmylation assays

Advanced Research Challenges

How to resolve contradictory UFC1 localization data across studies?

Observation	Potential Resolution	Supporting Evidence
Nuclear vs. cytoplasmic staining	Check cell cycle phase (G0/G1 arrest alters localization)	Cervical cancer models
Variable ER association	Monitor ER stress conditions	Reticulophagy studies
Discrepant molecular weights	Test for post-translational modifications	Ubiquitination assays

What methods confirm UFC1's functional role in DNA damage response?

Combine siRNA knockdown with γH2AX foci quantification
Perform ChIP-seq to map UFC1-UFM1 interaction sites on histone H4
Use ATM kinase inhibitors to dissect signaling pathways

How to design cross-species UFC1 studies given antibody reactivity limitations?

Species	Reactivity Confirmation	Experimental Validation
Mouse	Epitope alignment (87% homology)	Embryonic fibroblast models
Rat	C-terminal sequence verification	Primary neuron cultures
Primate	Phosphorylation site analysis	HEK293T transfection

Methodological Considerations

What quantitative approaches suit UFC1 expression analysis in clinical samples?

Digital droplet PCR for serum exosome UFC1 detection (AUC = 0.794)
Multiplex immunohistochemistry with PTEN/ATM pathway markers
Mass spectrometry with heavy isotope-labeled UFM1 peptides

How to address non-specific binding in UFC1 protein interaction studies?

Implement crosslinker gradients (0.1-2% formaldehyde)
Use size-exclusion chromatography pre-IP to remove aggregates
Validate interactions via NMR chemical shift perturbation assays

Critical Data Interpretation

Why do UFC1 knockdown results vary across cancer models?

Cancer Type	Observed Phenotype	Proposed Mechanism
NSCLC	Reduced proliferation (p<0.01)	PTEN epigenetic regulation
Cervical	G0/G1 arrest (p<0.05)	FOXP3 interaction loss
Leukemia	No apoptosis change	Alternative E3 ligase compensation

How to validate UFC1's role in ribosome quality control?

Perform ribosome profiling with harringtonine treatment
Conduct cryo-EM of 60S subunits from UFC1-deficient cells
Monitor UFM1-RPL26 conjugation via non-reducing PAGE

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Source

Quantity&Size*

Product Name

Message

ufc-1 Antibody