UNC45A Antibody
Description
Proteintech UNC45A Antibodies
These antibodies are critical for detecting UNC45A in cancer biology studies, particularly in breast, ovarian, and cervical cancers .
Key Research Applications
UNC45A antibodies have enabled breakthroughs in understanding its roles in:
Cancer Cell Proliferation and Mitotic Regulation
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Silencing UNC45A via siRNA inhibits breast cancer cell growth in vitro and tumor growth in vivo (Fig. 1E–G) .
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UNC45A localizes to centrosomes, where it regulates checkpoint kinase 1 (ChK1) activation and centrosomal separation .
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Depletion of UNC45A induces metaphase arrest, cytokinesis failure, and mitotic catastrophe due to disrupted NEK7 expression .
Actomyosin and Microtubule Dynamics
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UNC45A promotes myosin II folding and stress fiber assembly, with knockout cells showing defective contractile actomyosin bundles .
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It destabilizes microtubules (MTs) and is overexpressed in paclitaxel-resistant ovarian cancers .
Tissue-Specific Expression
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High UNC45A expression is observed in proliferative cells of the mouse ovaries, fallopian tube cilia, and neurons (Fig. 1B–C, 5A–G) .
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In human cancers, UNC45A correlates with tumor grade and chemoresistance .
Transcriptional Regulation
UNC45A localizes to the nucleus and enhances glucocorticoid receptor (GR)-mediated transcription of NEK7, a mitotic kinase essential for centrosome homeostasis (Fig. 2A, Table S1) .
Centrosomal Dysfunction
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UNC45A co-purifies with γ-tubulin and Hsp90β in centrosomal fractions (Fig. 2B–D) .
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Loss of UNC45A reduces ChK1 phosphorylation (S345) and centrosomal retention, leading to multi-nucleation (Fig. 4A–D) .
Therapeutic Targeting
UNC45A is a promising target for solid tumors, as its inhibition triggers apoptosis in HeLa and MDA-MB-231 cells in vivo (Fig. 1G–J) .
Validation Data and Citations
Future Directions
Product Specs
Target Background
- Whole-exome sequencing revealed that biallelic mutations in Unc-45 Myosin Chaperone A (UNC45A) are likely responsible for cholestasis, congenital diarrhea, impaired hearing, and bone fragility in affected individuals. PMID: 29429573
- UNC-45a promotes the generation of contractile actomyosin bundles by synchronizing non-muscle myosin II folding and filament assembly activities. PMID: 29055011
- Research findings provide valuable insights into the molecular mechanisms of neurite growth and identify UNC-45A as a critical regulator of NMII-mediated cellular processes in neurons. PMID: 28356421
- UNC-45A is a crucial component in regulating human NK cell cytoskeletal dynamics by facilitating the formation of actomyosin complexes. PMID: 26438524
- Research findings identify a novel centrosomal function for UNC45A and its role in cell proliferation and tumorigenesis. PMID: 25444911
- Researchers discovered that UNC-45A is expressed at the mRNA and protein levels as two isoforms. These isoforms differ only by a proline-rich 15-amino-acid sequence near the amino-terminus. PMID: 21802425
- GCUNC-45 is a novel modulator of progesterone receptor chaperoning by hsp90. PMID: 16478993
- Elevated GC UNC-45 protein expression is observed in ovarian carcinoma proliferation and metastasis. PMID: 17872978
- GCUNC45 is required for the normal cellular distribution of Hsp90beta, but not Hsp90alpha. PMID: 18285346
