UNC5D Antibody, HRP conjugated

Definition and Target Specificity

UNC5D (Unc-5 Homolog D) is a transmembrane receptor for netrins, notably NTN4, involved in axon guidance, cell migration, and apoptosis regulation . The HRP-conjugated UNC5D antibody targets specific epitopes, such as the central region (AA 90–212) , facilitating its use in quantitative assays.

Antibody Characteristics

Target Biology

• Function: Mediates axon repulsion, cell adhesion, and apoptosis in the absence of netrin ligands .

• Pathological Relevance: Downregulated in colorectal, breast, and lung cancers, suggesting a tumor-suppressive role .

Primary Uses

• ELISA: Detects UNC5D in serum or cell lysates with a recommended dilution of 1:500–1:1000 .

• Western Blot: Identifies UNC5D at ~105.9 kDa in human tissues .

Secondary Applications

• Immunohistochemistry (IHC): Localizes UNC5D in formalin-fixed paraffin-embedded tissues .

• Cell Migration Studies: Investigates UNC5D’s role in cancer metastasis .

Key Studies

• Neuronal Development: UNC5D guides axon growth cones during embryogenesis, as shown in knockout mouse models .

• Cancer Research: Reduced UNC5D expression correlates with poor prognosis in colorectal cancer .

• Apoptosis Regulation: UNC5D induces caspase-dependent cell death in the absence of netrin-1 .

Performance Metrics

Validation and Quality Control

• Purity: >95% by SDS-PAGE .

• Lot-Specific Data: Available upon request (e.g., binding affinity curves) .