unc-83 Antibody
Description
Overview of UNC-83 Antibody
The unc-83 antibody targets the UNC-83 protein, a transmembrane component of the nuclear envelope critical for nuclear migration in developing tissues. UNC-83 is expressed in a tissue-specific manner and localizes to migratory nuclei during embryogenesis . The antibody was generated by immunizing mice with an UNC-83/MBP fusion protein, followed by hybridoma screening .
2.1. Localization and Expression
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Nuclear Envelope Specificity: UNC-83 antibodies co-localize with lamin and UNC-84 at the nuclear envelope but are restricted to migratory nuclei (e.g., hyp7 precursors, P-cells, and intestinal cells) .
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Temporal Expression: First detected in hyp7 nuclei during embryogenesis, with later expression in P-cells and intestinal cells .
Table 1: UNC-83 Antibody Localization Patterns
| Tissue/Cell Type | Localization Pattern | Developmental Stage |
|---|---|---|
| Hyp7 precursors | Nuclear envelope | Embryogenesis |
| P-cells | Nuclear envelope | Larval development |
| Intestinal cells | Nuclear envelope | Post-embryonic |
2.2. Functional Insights
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Role in Nuclear Migration: UNC-83 is essential for nuclear migration in multiple tissues. Mutations in unc-83 disrupt nuclear positioning without affecting centrosome-nucleus attachment or lamin structure .
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Interaction with UNC-84: UNC-83 requires UNC-84 (a SUN-domain protein) for nuclear envelope localization. The physical interaction between UNC-83's KASH domain and UNC-84's SUN domain bridges the inner and outer nuclear membranes .
Table 2: UNC-83 Domain Architecture
2.3. In Vitro and In Vivo Studies
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Mammalian Cell Transfection: UNC-83 localizes to the outer nuclear membrane (ONM) when expressed in HEK 293 cells, while UNC-84 resides in the inner nuclear membrane (INM). This conserved targeting mechanism validates the antibody's cross-species utility .
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Co-Immunoprecipitation: UNC-83 interacts directly with kinesin light chain-2 (KLC-2), linking nuclei to microtubule motors for migration .
Table 3: Key UNC-83 Antibody Applications
Recent Advances (2025)
A 2025 preprint revealed isoform-specific roles for UNC-83:
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UNC-83a/b: Inhibit kinesin-1 to enable dynein-driven P-cell nuclear migration .
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UNC-83c: Activates kinesin-1 for hyp7 nuclear migration, with ectopic expression disrupting dynein function .
Table 4: UNC-83 Isoform Functions
| Isoform | Expression Context | Motor Regulation | Phenotype in Mutants |
|---|---|---|---|
| UNC-83a/b | P-cells | Inhibits kinesin-1 | Failed posterior migration |
| UNC-83c | Hyp7 cells | Activates kinesin-1 | Dorsal migration defects |
Validation and Limitations
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- UNC-83 acts as the cargo-specific adaptor between the outer nuclear membrane and the microtubule motors kinesin-1 and dynein. PMID: 20005871
- UNC-83 and UNC-84, the KASH and SUN domain proteins, bridge the nuclear envelope, connecting the nuclear lamina to cytoskeletal components. PMID: 16481402
