usp108 Antibody

USP10 Antibody and Its Role

• Function: USP10 stabilizes β-Catenin in APC-truncated colorectal cancer (CRC) cells by preventing its degradation, promoting WNT signaling and cancer stemness .

• Antibody Characteristics:

  • Reactivity: Human, mouse .

  • Applications: Western blot (WB), immunofluorescence (IF), immunoprecipitation (IP) .

  • Source: Rabbit recombinant or rabbit polyclonal .

  • Molecular Weight: ~110 kDa .

USP8 Antibody and Applications

• Function: USP8 regulates DNA repair and mitophagy through ubiquitin removal .

• Antibody Specifications:

  • Reactivity: Human, mouse .

  • Applications: WB, IP .

  • Source: Mouse monoclonal , sheep polyclonal , or rabbit monoclonal .

  • Molecular Weight: ~130 kDa .

Biological Context of USP10 and USP8

• USP10:

  • Cancer: Drives CRC progression by stabilizing β-Catenin, correlating with poor prognosis .

  • Immunity: Regulates B cell activation-induced cytidine deaminase (AID) stability, impacting antibody class-switching and affinity maturation .

• USP8:

  • DNA Repair: Deubiquitinates proteins like FANCI to maintain genome stability .

  • Mitochondrial Quality Control: Modulates Parkin-mediated mitophagy .

Antibody Validation and Use Cases

• USP10:

  • Validated for WB and IF to study CRC stemness .

  • Used in patient-derived organoid models to assess therapeutic targeting .

• USP8:

  • Employed in WB to detect USP8 in leukemia and osteosarcoma cell lines .

  • Applied in IP to study protein-protein interactions in DNA repair pathways .

Clinical Relevance

• USP10:

  • High expression correlates with aggressive CRC phenotypes .

  • Targeting USP10 may inhibit NOTUM signaling and tumor growth .

• USP8:

  • Overexpression linked to resistance to targeted therapies (e.g., PARP inhibitors) .