USP20 Antibody, HRP conjugated
Description
USP20 Antibody Characteristics
Key features of commercially available USP20 antibodies include:
HRP Conjugation in Antibody Detection
HRP-conjugated secondary antibodies amplify signals in assays by catalyzing chemiluminescent or chromogenic reactions. Key considerations:
| Application | HRP Substrate Type | Detection Method |
|---|---|---|
| Western Blotting | Chemiluminescent (e.g., ECL) | Light emission captured via imaging |
| Immunohistochemistry | Chromogenic (e.g., DAB) | Colored precipitate visible under microscopy |
| ELISA | Fluorescent/Colorimetric | Quantitative plate reader analysis |
HRP-based systems are preferred for high sensitivity and compatibility with diverse substrates .
DNA Damage Response
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USP20 stabilizes Rad17 by deubiquitination, enabling checkpoint kinase 1 (Chk1) activation and DNA repair. Depletion of USP20 increases Rad17 ubiquitination and impairs Chk1 phosphorylation .
Lipid Metabolism
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USP20 interacts with HSPA2, reducing its ubiquitination and stabilizing it to promote lipid accumulation in hepatocytes. Knockdown of USP20 decreases lipid content in HepG2 cells .
Reticulophagy Regulation
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USP20 deubiquitinates RETREG1, a receptor for ER-phagy, enhancing its stability. Inhibition of USP20 elevates RETREG1 ubiquitination, disrupting ER homeostasis .
NF-κB Signaling
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USP20 stabilizes p62, promoting PKCζ-mediated NF-κB activation during TNFα signaling. USP20 depletion reduces NF-κB target gene expression (e.g., BFL1, cFLIP) .
Cancer Metastasis
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USP20 stabilizes SNAI2, a transcription factor driving epithelial-mesenchymal transition (EMT) in breast cancer. USP20 overexpression correlates with lymph node metastasis and poor prognosis .
Western Blotting
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Primary Antibody: USP20 antibody (1:500–1:1000 dilution in blocking buffer).
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Secondary Antibody: HRP-conjugated anti-rabbit IgG (1:10,000 dilution).
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Detection: Chemiluminescent substrate (e.g., ECL) followed by imaging .
Immunoprecipitation
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Use 0.5–4.0 µg of USP20 antibody per 1.0–3.0 mg of lysate. Pre-clear lysates with protein A/G beads before adding the antibody .
Validation Data and Key Findings
Limitations and Considerations
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- USP20 regulates actin cytoskeleton organization and cell migration in a manner dependent on ERK3 expression. These findings identify USP20 as a bona fide regulator of ERK3 stability and physiological functions. PMID: 28167606
- Research has investigated the interaction between PKM2 and USP20. The results suggest a novel molecular pathway in cancer metabolism through the regulation of PKM2. PMID: 25708858
- Studies have revealed a dynamic regulation of USP20 by site-specific phosphorylation, highlighting the interdependence of signal transduction and trafficking pathways in balancing adrenergic stimulation and maintaining cellular homeostasis. PMID: 25666616
- Data establish a novel function of USP20 in genome maintenance and DNA repair. PMID: 24923443
- Analysis of indel variations in the human disease-associated genes CDKN2AIP, WDR66, USP20 and OR7C2 in a Korean population. PMID: 22552337
- Results indicate that USP20 is a key negative regulator of HTLV-1 Tax-induced NF-kappaB signaling. PMID: 21525354
- Identification and description of VDU2 in human cells. PMID: 12865408
- von Hippel-Lindau protein-interacting deubiquitinating enzyme 2 (VDU2) deubiquitinates and stabilizes HIF-1alpha. PMID: 15776016
- USPs 20 and 33 serve as novel regulators that dictate both post-endocytic sorting as well as the intensity and extent of beta(2)AR signaling from the cell surface. PMID: 19424180
