VAM3 Antibody

Biological Roles of VAMP3

VAMP3 facilitates membrane fusion in diverse cellular processes:

• Endosomal Recycling: Mediates recycling of transferrin receptors to the plasma membrane .

• Platelet Function: Required for α-granule secretion during platelet activation .

• Immune Response: Regulates T-cell receptor recycling to immunological synapses .

• Cell Migration: Coordinates membrane trafficking during motility .

Mechanistic Insights

• VAMP3 knockout studies revealed impaired granule secretion in platelets, linking it to hemostasis and thrombosis .

• In T-cells, VAMP3 depletion disrupted receptor recycling, impairing immune synapse formation .

Disease Associations

• Cancer: Elevated VAMP3 expression correlates with metastatic potential in breast cancer models .

• Neurological Disorders: Altered VAMP3 levels are observed in synaptic dysfunction pathologies .

Validation and Technical Considerations

• Western Blot: Detects endogenous VAMP3 at ~13 kDa in human, mouse, and rat lysates .

• Immunoprecipitation: Efficiently isolates VAMP3 complexes for interactome studies .

• Storage: Stable at -20°C for 12 months; avoid freeze-thaw cycles .

Key Citations

• McMahon et al. (1993): Identified VAMP3’s role in synaptic vesicle docking .

• Das et al. (2004): Demonstrated its necessity in T-cell receptor recycling .