VEGFB Antibody, HRP conjugated
Description
Definition and Basic Characteristics
VEGFB Antibody, HRP conjugated refers to a monoclonal or polyclonal antibody targeting vascular endothelial growth factor B (VEGFB), covalently linked to horseradish peroxidase (HRP). This conjugation enables enzymatic detection in assays such as Western blotting (WB) and ELISA. While VEGFB antibodies are utilized in research to study angiogenesis, tumor vasculature, and endothelial function, HRP-conjugated variants specifically amplify signal detection for quantitative analysis.
Key Features:
| Parameter | Details |
|---|---|
| Target | VEGFB (Vascular Endothelial Growth Factor B) |
| Conjugation | HRP enzyme for colorimetric or chemiluminescent signal amplification |
| Applications | Western blotting, ELISA, immunohistochemistry (IHC) |
| Reactivity | Primarily human, with cross-reactivity reported in murine and rat models |
| Isoforms Detected | VEGFB 167 and VEGFB 186 (depending on antibody specificity) |
Mechanism of Action and Research Applications
VEGFB antibodies bind to VEGFB, a growth factor involved in angiogenesis and endothelial survival. HRP conjugation facilitates downstream detection via substrate conversion (e.g., TMB for colorimetric assays).
Critical Roles of VEGFB in Physiology and Pathology
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Angiogenesis Regulation: VEGFB can inhibit excessive angiogenesis by binding FGFR1/VEGFR1 heterodimers, suppressing FGF2-driven signaling in tumors .
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Tumor Vasculature: Anti-VEGF-B antibodies (e.g., 2H10) normalize aberrant tumor blood vessels, improving chemotherapy efficacy .
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Cardioprotection: VEGFB gene therapy prevents doxorubicin-induced cardiotoxicity by preserving cardiac microvasculature .
Comparison of VEGFB Antibodies and HRP-Conjugated VEGF Antibodies
While VEGFB-specific HRP-conjugated antibodies are less commonly documented in literature, VEGFA (VEGF-A) HRP antibodies are widely used. Below is a comparative analysis:
VEGFB’s Dual Role in Angiogenesis
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Anti-Angiogenic Effect: VEGFB binds FGFR1, forming FGFR1/VEGFR1 complexes that suppress FGF2-induced ERK activation, limiting excessive tumor angiogenesis .
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Pro-Angiogenic Activity: In Matrigel assays, VEGFB 167 promotes endothelial cell ingrowth via VEGFR1 signaling, enhancing Akt and eNOS activation .
Therapeutic Implications
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Anti-VEGF-B Antibodies: 2H10 reduces abnormal tumor vasculature, improving chemotherapy response without affecting normal tissue .
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Gene Therapy: Adeno-associated viral VEGFB delivery prevents doxorubicin-induced cardiac atrophy and mitochondrial dysfunction .
Challenges and Future Directions
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Limited Commercial Availability: HRP-conjugated VEGFB antibodies are not prominently featured in catalogs; most HRP antibodies target VEGFA .
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Mechanistic Complexity: VEGFB’s context-dependent roles (inhibitory vs. stimulatory) necessitate isoform-specific antibodies for precise research .
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Translational Potential: Anti-VEGF-B therapies may complement anti-VEGFA treatments in oncology, though clinical validation is pending .
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Elevated plasma VEGF-B levels are associated with type 2 diabetes mellitus. PMID: 28523459
- Clinical studies indicate alterations in circulating or tissue expression levels of VEGF-B in obese individuals compared to lean individuals. PMID: 28798193
- Cardiac transgenic overexpression of vascular endothelial growth factor-B did not protect heart transplants from ischemia-reperfusion injury. PMID: 27588416
- Renal VEGF-B expression correlates with the severity of Diabetic Kidney Disease. PMID: 28190774
- Studies show that metformin treatment reduces serum vascular endothelial growth factor B (VEGF-B) levels and improves insulin resistance. PMID: 26387747
- Frameshift mutations in the VEGFB gene are linked to stomach and colorectal cancers. PMID: 25633991
- Fluid shear stress stimulates the synthesis of Insulin growth factor-2 and vascular endothelial growth factors (VEGF) B and D, which in turn activate MMP-12. PMID: 25435370
- MMP9 can activate VEGF-B through the PI3K/Akt signaling pathway. PMID: 25424698
- Vascular endothelial growth factor plays a role in amyotrophic lateral sclerosis. PMID: 24987705
- Low VEGFB and VEGFD gene expression is associated with early-stage non-small cell lung cancer. PMID: 24145997
- Research suggests that VEGF-B is an angiogenesis factor in vitro, and its activity is mediated by ERK1/2 and p38-related signaling pathways. PMID: 24374930
- VEGF-B potentially plays roles in cardiac protection, energy metabolism support, and neuroprotection [review]. PMID: 24987005
- High VEGF-B levels may correlate with the presence of hyperlipidemia and target organ damage in type 2 diabetic patients. PMID: 25001655
- High VEGFB expression is associated with bone marrow metastasis in neuroblastoma. PMID: 23553333
- VEGF-B may be a critical ligand in signaling between the tumor and pre-existing blood vessels, ensuring a functional blood supply for tumor survival. PMID: 23417498
- There are significant associations between certain VEGF (VEGF-B) family members and overall survival and distant failure. PMID: 23728940
- Data indicate that three microRNAs (miR-484, -642, and -217) can predict chemoresistance and vasculature of serous epithelial ovarian carcinomas by regulating the VEGFB and VEGFR2 pathways. PMID: 23697367
- Expression of VEGF-B genes in glioma cell lines U87 is significantly altered under hypoxic and ischemic conditions. PMID: 23350126
- In WT1 mutant cells, reduced VEGF(165)b is due to a lack of WT1-mediated transcriptional repression of the splicing-factor kinase SRPK1. PMID: 22172722
- Analysis of vascular endothelial growth factor-B binding by VEGFR-1(D2). PMID: 20501651
- TIMP3 blocks the binding of VEGF to VEGF receptor-2, inhibiting downstream signaling and angiogenesis. PMID: 12652295
- The crystal structure of human vascular endothelial growth factor-B (VEGF-B) is described, and a predicted model for the association of VEGF-B with the second domain of its receptor, VEGFR-1, is presented. PMID: 16616187
- Basophils may play a role in angiogenesis and inflammation through the expression of various forms of VEGF-B and their receptors. PMID: 17082651
- VEGFB and its receptor are highly expressed in dysplastic neurons. Immunoreactivity (IR) in astroglial and balloon cells is observed for VEGFA and its receptors. Double-labeling also shows expression of VEGFA, VEGFB, and VEGFR-1 in cells of the microglia/macrophage lineage. PMID: 18317782
- VEGF-B appears to have a relatively limited angiogenic activity in the ischemic heart. PMID: 18511699
- Increased VEGFB expression is associated with hepatocellular carcinoma. PMID: 18537151
- Overexpression of vascular endothelial growth factor-B in the mouse heart alters cardiac lipid metabolism and induces myocardial hypertrophy. PMID: 18757827
- The structural features of the 'highly ordered' interaction of the Fab fragment of the antibody (Fab-2H10) with VEGF-B are presented. PMID: 18930733
- VEGF-B mRNA was not expressed in either normal urothelium or bladder cancer. PMID: 19424629
