slc17a6a Antibody

Introduction

The SLC17A6a antibody is a research tool designed to detect the SLC17A6a protein, a member of the solute carrier family 17. This protein functions primarily as a vesicular glutamate transporter, playing a critical role in neurotransmitter loading into synaptic vesicles at excitatory synapses . The antibody is widely used in neurobiology and molecular biology studies to investigate glutamatergic signaling and synaptic plasticity.

Antibody Structure and Specificity

The SLC17A6a antibody targets epitopes within the cytoplasmic C-terminus of the SLC17A6a protein. Two prominent variants are available:

2.1. Mouse Monoclonal Antibody (Catalog No. ABIN2483748)

• Immunogen: Fusion protein spanning amino acids 501–582 of rat VGlut2 .

• Reactivity: Cross-reacts with human, mouse, and rat proteins .

• Applications: Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) .

• Conjugation: Available in PE, HRP, and fluorescent formats for multiplex assays .

2.2. Rabbit Polyclonal Antibody (Catalog No. ABIN6242117)

• Immunogen: Synthetic peptide corresponding to amino acids 574–607 of zebrafish SLC17A6a .

• Reactivity: Specific to zebrafish (Danio rerio) .

• Applications: Western blotting (WB) for tissue lysate analysis .

• Purification: Protein A and peptide affinity chromatography .

3.1. Neurotransmitter Studies

The SLC17A6a antibody is essential for studying glutamatergic synaptic transmission. It detects vesicular glutamate transporters in excitatory neurons, enabling researchers to:

• Investigate synaptic vesicle dynamics .

• Analyze neurotransmitter release mechanisms in brain lysates .

3.2. Comparative Models

• Zebrafish Studies: The rabbit polyclonal antibody facilitates developmental neuroscience research in zebrafish, tracking SLC17A6a expression during synapse formation .

• Rodent Models: The mouse monoclonal variant is validated for rat brain lysates, supporting cross-species comparative analyses .

Methodological Advancements

Recent advancements in antibody screening, such as next-generation sequencing (NGS)-linked functional assays, have streamlined the production of high-affinity antibodies like SLC17A6a . These methods combine:

• Single-cell isolation to identify antigen-specific B cells.

• Golden Gate Cloning for rapid dual Ig expression vectors .

• Flow cytometry to enrich high-affinity clones .