vglu-3 Antibody

Biological Role of VGLUT3

VGLUT3 is a member of the solute carrier family 17 (SLC17A8) and is distinct from VGLUT1 and VGLUT2 in both expression patterns and functional roles . Key features include:

• Tissue Distribution: Expressed in brain regions (amygdala, hippocampus, hypothalamus), kidney, liver, and non-neuronal cells like podocytes .

• Neurological Functions: Found in atypical glutamatergic cells, including serotoninergic raphe neurons and dopaminergic substantia nigra neurons .

• Disease Associations: Linked to deafness (DFNA25) and implicated in cancer (ovarian, lung) .

Neuroscience Studies

• Glutamate Release: VGLUT3 knockdown in podocytes reduces high K⁺-induced glutamate release by 60% .

• Cochlear Trauma: VGLUT3 expression increases in auditory brainstem post-hearing loss .

• Co-Localization: Found in cholinergic (VAChT⁺) and monoaminergic (VMAT2⁺) synaptic vesicles .

Methodological Insights

• Immunocytochemistry: Optimal dilution ranges from 1:100 to 1:500 .

• Western Blot: Detects ~65 kDa band in rat brain lysates .

• Electron Microscopy: Immunogold staining localizes VGLUT3 to vesicle membranes in podocytes .

Key Research Findings

Technical Considerations

• Storage: Lyophilized antibodies stable at -20°C; reconstituted aliquots avoid freeze-thaw cycles .

• Validation: KO validation confirms specificity (e.g., SY-135203) .

• Limitations: Cross-reactivity with non-mammalian species (e.g., electric ray) reported in select clones .