Vimentin Antibody, Biotin
Description
Introduction
Vimentin Antibody, Biotin is a biotin-conjugated monoclonal or polyclonal antibody designed to detect vimentin, a type III intermediate filament protein expressed in mesenchymal cells. This antibody is widely used in immunohistochemistry (IHC), Western blotting (WB), and flow cytometry to study cell structure, migration, and pathological processes such as cancer metastasis and autoimmune diseases . Vimentin plays critical roles in cytoskeletal organization, immune responses, and extracellular signaling .
Key Features of Vimentin Antibody, Biotin
Immunohistochemistry (IHC)
Vimentin Antibody, Biotin is validated for detecting vimentin in formalin-fixed tissues (e.g., liver cancer, tonsillitis) with antigen retrieval using citrate (pH 6.0) or TE buffer (pH 9.0) . It serves as a marker for epithelial-to-mesenchymal transition (EMT) in tumors .
Angiogenesis Studies
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A phage-derived anti-vimentin antibody (LOB7) increased endothelial tube formation by 21% in 2D matrigel assays .
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Enhanced tube formation peaked at 5–10 hours post-treatment, suggesting transient modulation of extracellular vimentin interactions .
Autoimmune Disease Research
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Anti-acetylated vimentin antibodies are linked to early rheumatoid arthritis (RA), with 24% of primary Sjögren’s disease (SjD) patients showing elevated levels .
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Antibodies against post-translationally modified vimentin (citrullinated, carbamylated) correlate with disease severity .
Key Studies
Clinical Relevance
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Cancer Diagnostics: Vimentin expression in tumors (e.g., gliomas, colorectal carcinomas) predicts metastatic potential .
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Therapeutic Targeting: Antibodies like hzVSF are explored for HBV treatment, showing reduced viral entry in woodchuck models .
Challenges and Limitations
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Cross-Reactivity: Some antibodies show weak cross-reactivity with carbamylated or citrullinated vimentin isoforms .
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Batch Variability: Storage conditions (e.g., exposure to light) and lot-to-lot differences may affect performance .
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Functional Complexity: Extracellular vimentin’s role in angiogenesis and immunity remains incompletely understood .
Future Directions
Product Specs
Q&A
Vimentin Antibody, Biotin: Research-Focused FAQs
This curated FAQ addresses critical methodological and analytical challenges in working with biotin-conjugated vimentin antibodies, emphasizing experimental design, data interpretation, and advanced research applications.
Advanced Research Questions
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How to resolve contradictory findings on vimentin’s role in angiogenesis using biotinylated antibodies?
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What strategies minimize biotin-streptavidin interference in high-sensitivity immunoassays?
Methodological Challenges
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How to study extracellular vimentin interactions using biotin-labeled antibodies?
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Secretome analysis: Treat macrophages with TNF-α (10 ng/mL, 24 hr) to induce vimentin secretion, followed by immunoprecipitation with biotinylated antibodies and LC-MS/MS .
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Surface plasmon resonance (SPR): Immobilize recombinant vimentin on sensor chips to quantify binding kinetics (kon/koff) of biotinylated antibodies .
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What controls are critical for flow cytometry with biotinylated vimentin antibodies?
Data Interpretation
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How to distinguish nonspecific binding in extracellular vimentin studies?
Product Science Overview
Vimentin is a type III intermediate filament protein that is predominantly expressed in mesenchymal cells. It plays a crucial role in maintaining cell integrity, stabilizing cytoskeletal interactions, and supporting cellular processes such as migration, shape, and organelle positioning . The VIM gene, which encodes vimentin, is located on chromosome 10 in humans . Vimentin is often used as a marker to identify cells of mesenchymal origin and is involved in various biological processes, including intermediate filament organization, gene expression regulation, and cellular response to external stimuli .
Mouse anti-human antibodies are secondary antibodies produced by immunizing mice with human immunoglobulins. These antibodies are highly specific for human immunoglobulins and are commonly used in research and diagnostic applications to detect, sort, or purify human proteins . They are often conjugated with various labels, such as enzymes or fluorophores, to facilitate detection in techniques like ELISA, Western blotting, and immunohistochemistry . The development of mouse anti-human antibodies has significantly advanced biomedical research by enabling precise targeting and visualization of human proteins in various experimental settings .
Biotin, also known as vitamin B7 or vitamin H, is a water-soluble B-vitamin that plays a vital role in various metabolic processes. It acts as a coenzyme for carboxylase enzymes, which are involved in the synthesis of fatty acids, gluconeogenesis, and the catabolism of amino acids . Biotin is essential for maintaining healthy skin, hair, and nails, and it supports the normal functioning of the nervous system . It is found in a wide range of foods, including eggs, nuts, and certain vegetables, and is also produced by gut bacteria . Biotin deficiency is rare but can lead to symptoms such as hair loss, skin rashes, and neurological issues .
The combination of vimentin, mouse anti-human antibodies, and biotin is particularly useful in various research and diagnostic applications. For instance, biotinylated mouse anti-human vimentin antibodies can be used in immunoassays to detect vimentin expression in human tissues. This approach leverages the high specificity of mouse anti-human antibodies and the strong binding affinity of biotin to streptavidin, enabling sensitive and accurate detection of target proteins.
