VPS15 Antibody
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Product Specs
Buffer
**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
VPS15 antibody; At4g29380 antibody; F17A13.200 antibody; Serine/threonine-protein kinase VPS15 antibody; EC 2.7.11.1 antibody; Vacuolar protein sorting-associated protein 15 antibody; AtVPS15 antibody
Target Names
VPS15
Uniprot No.
Target Background
Function
VPS15 is a serine/threonine-protein kinase essential for cytoplasm to vacuole transport (Cvt) and autophagy. It functions as part of the autophagy-specific VPS34 PI3-kinase complex I. VPS15 plays a crucial role in pollen development and germination, likely through the regulation of phosphatidylinositol 3-phosphate (PI3P) formation and vacuolar organization.
Gene References Into Functions
- Research has demonstrated that AtVPS15 interacts directly with AtVPS34, indicating its importance in pollen germination potentially through the modulation of PI3-kinase activity. PMID: 22361507
- Evidence suggests that AtVPS15 (At4g29380; NP_194667.1) contributes to pollen development and germination by regulating PI3P biosynthesis in Arabidopsis. PMID: 21833541
Database Links
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Subcellular Location
Cytoplasm. Golgi apparatus, trans-Golgi network membrane; Lipid-anchor. Endosome membrane; Lipid-anchor.
Tissue Specificity
Mainly expressed in anthers, pollen grains and pollen tubes, and, to a lower extent, in other tissues and organs including seedlings, roots, stems, leaves, flowers, pitils and siliques.
Q&A
Here’s a structured collection of FAQs tailored for researchers working with VPS15 antibody in academic settings, incorporating methodological insights and data from peer-reviewed studies:
Advanced Research Questions
How to resolve contradictory data on VPS15’s role in mTORC1 regulation?
What methods confirm VPS15’s interaction with PI3K-III complex members?
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Co-IP protocols:
How to address unexpected ubiquitination signals in VPS15 blots?
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Treat cells with proteasome inhibitors (MG132) or lysosomal inhibitors (chloroquine) during CHX chase experiments .
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Perform Nedd4 knockdown to reduce K11-linked polyubiquitination of VPS15 .
Methodological Best Practices
Critical controls for VPS15 antibody-based assays
Quantitative analysis of VPS15-associated vesicles
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Use automated image analysis (e.g., CellProfiler) to quantify LC3/VPS15 vesicle colocalization .
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For PI3P quantification: Employ 2xFYVE-GFP reporters and measure fluorescence intensity after wortmannin treatment .
Data Interpretation Challenges
Discrepancies in autophagosome-lysosome fusion assays
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If Lamp2/VPS15 colocalization is inconsistent:
Validating antibody performance across species
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