VSR1 Antibody

Introduction to VSR1 Antibody

The VSR1 antibody is a polyclonal immunoglobulin developed to detect vacuolar sorting receptor 1 (VSR1), a key regulatory protein in plant cells responsible for sorting soluble cargo proteins from the trans-Golgi network to vacuoles. This antibody is widely used in plant biology research to study intracellular protein trafficking, particularly in model organisms like Arabidopsis thaliana and Nicotiana tabacum .

Key Features of VSR1 Antibody

• Host species: Rabbit

• Clonality: Polyclonal

• Reactivity: Arabidopsis thaliana, Nicotiana tabacum

• Applications:

  • Western blot (1:1,000–1:5,000 dilution)

  • Immunofluorescence (1:500 dilution)

• Immunogen: Recombinant His6-tagged VSR1 (amino acids 20–566 of Arabidopsis VSR1)

• Storage: -20°C in PBS with 50% glycerol

Molecular Role of VSR1

VSR1 facilitates the sorting of seed storage proteins (e.g., 12S globulin, 2S albumin) to protein storage vacuoles (PSVs) via clathrin-coated vesicles . It recognizes C-terminal vacuolar sorting determinants (ctVSDs) on cargo proteins, such as the SYGRPRVAAA motif in β-conglycinin . Mutations in VSR1 (e.g., R95M) disrupt cargo binding, leading to missorting and secretion of storage proteins .

Key Studies Using VSR1 Antibody

Functional Validation

• Genetic evidence: vsr1 mutants exhibit defective PSV formation and accumulate storage proteins in aberrant extracellular compartments .

• Biochemical evidence: VSR1 competes for binding between ctVSDs (e.g., CRU1) and N-terminal propeptide signals (e.g., aleurain-ssVSD) .

Antibody Performance

Limitations

• Not validated for immunoprecipitation or mass spectrometry .

• Potential cross-reactivity with other VSR isoforms (e.g., VSR2–VSR7) in Arabidopsis due to homology .