WDR24 Antibody, FITC conjugated

Validation and Quality Control

• Specificity: Validated using known positive controls (e.g., human 293T cells) and negative samples to confirm minimal cross-reactivity .

• Batch Consistency: Quality assurance includes SDS-PAGE and spectral analysis to ensure conjugate stability and lot-to-lot reproducibility.

• Performance: Optimal working concentrations must be determined by end users due to variability in sample preparation and equipment .

Immunofluorescence (IF)

• Protocol: Fixed cells or tissues are incubated with the FITC-conjugated antibody (suggested dilution: 1:50–1:200), followed by imaging using fluorescence microscopy.

• Example Data: In HeLa cells, WDR24 localizes to lysosomes and autolysosomes, consistent with its role in mTORC1 signaling and autophagy regulation .

ELISA

• Sensitivity: Detects WDR24 at concentrations as low as 0.1–0.5 µg/ml in sandwich ELISA setups .

Biological Context of WDR24

WDR24 is a component of the GATOR2 complex, which regulates mTORC1 signaling in response to amino acid availability. Key findings include:

• Functional Role: Facilitates lysosome acidification and autophagic flux independently of mTORC1 .

• Structural Insights: Interacts with Sestrin2 and Seh1L via its RING domain, critical for leucine sensing .

Comparative Analysis with Other WDR24 Antibodies

Limitations and Considerations

• No Published Data: As of March 2025, no peer-reviewed studies specifically using the FITC-conjugated variant have been published.

• Species Reactivity: Predicted reactivity in rodents requires empirical validation .

Procurement and Compliance

• Pricing: $166 (1 mL stock solution) .

• Regulatory: For research use only; not validated for clinical diagnostics .