WDR92 Antibody, FITC conjugated

Product Overview

The WDR92 antibody is typically a rabbit polyclonal immunoglobulin (IgG) raised against recombinant WDR92 protein or synthetic peptides corresponding to specific regions of the protein. FITC conjugation involves covalently linking the dye to lysine residues on the antibody, ensuring optimal fluorescence while preserving antigen-binding activity . Key product specifications include:

Applications in Research

The FITC-conjugated WDR92 antibody is employed in various experimental workflows to study protein localization, expression, and interactions:

• Immunofluorescence (IF):
  Detects WDR92 localization in fixed cells or tissues. For example, studies using this antibody have shown nuclear and cytoplasmic staining in human U-2 OS cells , consistent with WDR92’s roles in nuclear processes and cytoplasmic dynein assembly .

• Immunohistochemistry (IHC):
  Identifies WDR92 expression in paraffin-embedded tissues. Strong cytoplasmic and nuclear positivity has been observed in human stomach glandular cells .

• Western Blot (WB):
  Quantifies WDR92 levels in lysates. WB analysis of NIH-3T3 (mouse) and NBT-II (rat) cell lysates confirms cross-reactivity .

Research Findings and Biological Relevance

WDR92 is a conserved WD-repeat protein with critical roles in:

• Apoptosis: Overexpression induces pro-apoptotic effects in mammalian cells .

• Dynein Motor Assembly: Interacts with prefoldin-like complexes and dynein heavy chains (HCs) to stabilize axonemal dynein HC formation . Mutations in WDR92 disrupt HC assembly, leading to defects in ciliary and flagellar motility .

The antibody’s ability to specifically detect WDR92 has enabled studies linking it to:

• Cochaperone Complexes: WDR92 associates with the R2TP complex and prefoldin-like proteins to facilitate protein folding .

• Ciliary Dysmotility: Dysregulation of WDR92 contributes to ciliopathies, as evidenced by its interaction with dynein assembly factors (DNAAFs) .

Technical Considerations

• Conjugation Process: FITC labeling involves reacting the antibody with FITC isothiocyanate in anhydrous DMSO, typically at a ratio of 40–80 µg FITC/mg antibody . Optimal labeling balances fluorescence brightness and antibody solubility .

• Storage: Antibodies are stabilized in PBS with sodium azide and glycerol, stored at -20°C to prevent degradation .