RRS1 Antibody

Introduction

The RRS1 antibody is a specialized tool for detecting the ribosome biogenesis regulator 1 (RRS1), a nuclear protein critical for ribosome assembly and maturation. RRS1 is implicated in cancer progression, cell proliferation, and stress responses. This antibody is widely used in research to investigate RRS1's molecular mechanisms and its pathological roles, particularly in oncology .

2.2. Recommended Dilutions

Source: Proteintech (15329-1-AP) and Abcam (ab188161) .

Hepatocellular Carcinoma (HCC)

• Function: RRS1 promotes HCC growth by retaining RPL11 in the nucleolus, inhibiting its binding to MDM2, and destabilizing p53 .

• Evidence:

  • Knockdown of RRS1 reduces tumor growth in xenograft models .

  • Overexpression correlates with poor survival in HCC patients .

Breast Cancer

• Function:

  • Drives metastasis via the RPL11-c-Myc-SNAIL axis .

  • Stabilizes GRP78 to activate PI3K/AKT signaling .

• Evidence:

  • RRS1 knockdown reduces proliferation, migration, and invasion in BT549 and MDA-MB-231 cells .

  • High RRS1 expression correlates with lymph node metastasis .

Retinoblastoma (RB)

• Function: Activates AKT/mTOR signaling to enhance tumor proliferation .

• Evidence:

  • RRS1 overexpression increases phosphorylation of AKT and S6 in Y79 and WERI-Rb-1 cells .

3.2. Ribosome Biogenesis and Stress Response

• Mechanism:

  • Facilitates rRNA maturation (28S and 18S) and ribosome subunit assembly .

  • Retains RPL11 in the nucleolus, reducing ribosomal stress-induced p53 activation .

• Stress Response:

  • Induced by endoplasmic reticulum (ER) stress in neurodegenerative diseases .

Clinical Relevance

5.1. Ribosome Biogenesis

• Key Partners: RPF2, RPL11, and 5S RNP .

• Process:

  • RRS1 ensures proper 5S RNP integration into pre-60S ribosomal subunits .

  • Depletion disrupts ribosome assembly, triggering nucleolar stress .

5.2. Protein Stabilization

• GRP78 Interaction: RRS1 inhibits GRP78 ubiquitination, enhancing its stability and activating PI3K/AKT signaling .

• AEG-1 Interaction: Stabilizes AEG-1 to upregulate MDR1 and confer chemoresistance .

Future Directions

• Therapeutic Targeting: Small-molecule inhibitors disrupting RRS1-RPL11 interactions could reactivate p53 in HCC .

• Biomarker Potential: High RRS1 expression predicts poor prognosis in multiple cancers .

References

1. Proteintech. RRS1 Antibody (15329-1-AP).

2. Science Advances. Genomic gain of RRS1 promotes hepatocellular carcinoma (2021).

3. Spandidos Publications. Downregulated RRS1 inhibits breast cancer metastasis (2022).

4. PMC. RRS1 promotes retinoblastoma progression (2020).

5. PMC. RRS1 in breast cancer proliferation (2018).

6. Abcam. Anti-RRS1 antibody (ab188161).