XTH8 Antibody

Target Protein (XTH8)

• Function: Modifies xyloglucan-cellulose networks to regulate cell wall plasticity during plant development and abiotic stress responses .

• Molecular Weight: ~31–33 kDa (inferred from homologous XTH enzymes) .

• Catalytic Activity: Exhibits dual endotransglucosylase/hydrolase activity, enabling structural remodeling of cell wall polysaccharides .

Antibody Properties

• Host Species: Rabbit (polyclonal, inferred from similar XTH-targeting antibodies) .

• Applications: Western blot (WB), ELISA, and immunoassays for quantifying XTH8 expression .

• Immunogen: Likely derived from conserved peptide sequences within XTH8’s catalytic domain .

Key Applications

• Expression Profiling: Used to study XTH8 upregulation under stress conditions (e.g., drought, salinity) .

• Subcellular Localization: Identifies XTH8 in cell walls and vascular tissues via immunohistochemistry .

• Functional Studies: Links XTH8 activity to enhanced mechanical strength in plant tissues .

Experimental Data

Table 1: XTH8 Expression Under Abiotic Stress

Comparative Analysis with Related Antibodies

Table 2: XTH-Targeting Antibodies

Technical Considerations

• Cross-Reactivity: May recognize conserved epitopes across XTH isoforms .

• Validation: Requires knockout mutants or peptide-blocking assays to confirm specificity .

• Storage: Lyophilized formulations recommended for long-term stability .

Challenges and Future Directions

• Limited Commercial Availability: Few antibodies specifically targeting XTH8 are documented, necessitating custom development .

• Functional Redundancy: Overlapping roles of XTH isoforms complicate phenotype interpretation .

• Therapeutic Potential: Engineered XTH8 inhibitors could enhance crop resilience to environmental stress .