At1g11050 Antibody

Definition and Target Protein

The At1g11050 antibody specifically binds to the protein product of the At1g11050 gene, which encodes a serine/threonine protein kinase (UniProt ID: O04086) . This kinase is implicated in intracellular signaling cascades, particularly under oxidative stress conditions .

Role in Oxidative Stress Response

• In a transcriptomic study of Arabidopsis cell cultures exposed to high-light (HL) stress, At1g11050 was upregulated with a log₂ fold change of 1.21 after 30 minutes of HL treatment .

• The kinase is associated with reactive oxygen species (ROS) signaling, specifically linked to singlet oxygen ($^1\text{O}_2$) and hydrogen peroxide ($\text{H}_2\text{O}_2$) pathways (Table 1) .

Mechanistic Insights

• The kinase likely participates in phosphorylation events that modulate stress-responsive transcription factors or repair enzymes during photooxidative damage .

• Its upregulation under HL suggests a role in mitigating ROS-induced cellular damage, potentially through activation of downstream defense genes .

Functional Context in Arabidopsis

• Biological Process: Involved in stress-activated kinase signaling, defense response to oxidative stress, and phosphorylation-mediated signal transduction .

• Cellular Localization: Predicted to function in the cytoplasm or nucleus based on kinase activity and interaction partners.

Research Applications

• Western Blotting: Used to detect At1g11050 protein expression under stress conditions.

• Functional Studies: Employed to elucidate kinase-substrate relationships in ROS signaling pathways.

• Comparative Analyses: Contrasted with other ROS-responsive markers (e.g., At4g10500 for $\text{H}_2\text{O}_2$) to map stress-specific transcriptional networks .

Limitations and Future Directions