At1g31060 Antibody

Hypothetical Use Cases

Though no direct studies on At1g31060 are cited in the provided sources, potential applications align with common antibody uses in plant biology:

1. Protein Localization: Identifying subcellular compartments where Q6NLU6 is expressed.

2. Gene Expression Analysis: Quantifying protein levels during developmental stages or stress responses.

3. Interaction Mapping: Co-immunoprecipitation (Co-IP) to identify protein complexes.

Validation Considerations

Critical validation steps for antibodies include:

• Specificity: Ensuring no cross-reactivity with non-target proteins.

• Sensitivity: Detecting low-abundance targets reliably.

• Reproducibility: Consistent performance across experimental batches.

Caveats: Commercial antibodies, including those targeting plant proteins, may lack rigorous validation. For example, studies on angiotensin II type 1 (AT1) receptor antibodies in humans highlight non-specific binding and variable performance across suppliers . While this pertains to mammalian systems, similar scrutiny is essential for plant antibodies like At1g31060.

Comparative Analysis with Related Arabidopsis Antibodies

The table below contrasts At1g31060 with other Arabidopsis-specific antibodies listed in the Cusabio catalog :

Key Observations:

• All antibodies target Arabidopsis proteins with diverse UniProt IDs.

• No functional redundancy is evident from the provided data.

Recommendations for Further Research

1. Epitope Mapping: Define the antigenic region recognized by At1g31060 to assess cross-reactivity.

2. Validation Protocols: Use knockout Arabidopsis lines or competitive peptide inhibition assays to confirm specificity .

3. Collaborative Studies: Partner with plant biology laboratories to publish application-specific data.