At1g61460 Antibody
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Target Background
Q&A
Here’s a structured FAQ collection for researchers working with At1g61460 Antibody, integrating technical specifications and methodological insights from peer-reviewed data:
What experimental applications are suitable for At1g61460 antibodies in plant molecular studies?
Core Applications
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Protein Localization: Immunofluorescence in Arabidopsis root or leaf tissues to study subcellular distribution .
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Kinase Activity Assays: Co-immunoprecipitation (Co-IP) with downstream substrates to map signaling pathways.
Advanced Functional Studies
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Stress Response Profiling: Quantify At1g61460 expression under abiotic stress (e.g., salinity, drought) via ELISA or WB .
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Protein-Protein Interaction Networks: Combine Co-IP with mass spectrometry to identify novel binding partners.
How to address cross-reactivity issues in complex plant protein extracts?
Methodological Solutions
| Strategy | Rationale | |
|---|---|---|
| Pre-Absorption | Incubate antibody with recombinant At1g61460 protein before application | Saturates antibody binding sites, eliminating non-specific signals |
| Tagged Protein Rescue | Express HA/FLAG-tagged At1g61460 in knockout lines | Distinguishes endogenous vs. exogenous protein in WB/IF |
What controls are essential for ensuring reproducibility?
Critical Controls
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Positive Control: Arabidopsis tissue overexpressing At1g61460 (e.g., transgenic lines).
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Technical Replicates: Triplicate WB/ELISA runs to assess inter-assay variability.
How to resolve conflicting data on At1g61460 expression across studies?
Data Contradiction Analysis
| Factor | Impact | Mitigation Strategy |
|---|---|---|
| Tissue-Specific Isoforms | Alternative splicing in roots vs. leaves | Use isoform-specific primers/probes in RT-qPCR |
| Post-Translational Modifications | Phosphorylation alters antibody binding | Treat extracts with phosphatases pre-WB |
| Growth Conditions | Light/temperature stress alters protein levels | Standardize growth protocols across labs |
Technical Specifications Table
| Parameter | Value | Source |
|---|---|---|
| Target Gene | AT1G61460 (G-type lectin S-receptor-like kinase) | |
| Antibody Purity | ≥90% (SDS-PAGE) | |
| ELISA Titer | 1:64,000 | |
| Cross-Reactivity | Untested with non-Arabidopsis species |
