At1g19565 Antibody
Description
Introduction to At1g19565 Antibody
The At1g19565 Antibody is a polyclonal antibody specifically designed to recognize the protein encoded by the At1g19565 gene in Arabidopsis thaliana (mouse-ear cress). This antibody serves as a critical tool in plant biology research, enabling the detection and study of the At1g19565 protein in various experimental contexts. While limited peer-reviewed studies explicitly detail its applications, product specifications and technical data provide insights into its utility for researchers investigating Arabidopsis gene function, protein localization, and downstream cellular processes.
Applications in Research
The At1g19565 Antibody is validated for ELISA and Western Blot (WB) assays, making it suitable for detecting the At1g19565 protein in Arabidopsis samples. Potential research applications include:
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Protein Expression Analysis: Quantifying At1g19565 protein levels under varying growth conditions or genetic backgrounds.
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Subcellular Localization: Identifying the intracellular compartment (e.g., cytoplasm, nucleus) where At1g19565 is localized using immunoblotting or immunoprecipitation (IP).
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Functional Studies: Investigating the role of At1g19565 in signaling pathways, stress responses, or developmental processes.
Limitations
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Species Specificity: Primarily validated for Arabidopsis thaliana; cross-reactivity with other plant species or organisms is unconfirmed.
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Experimental Validation: Users must optimize antibody dilutions and blocking conditions for their specific assays, as no standardized protocols are provided.
Research Context and Gaps
The At1g19565 Antibody is a specialized reagent for Arabidopsis research, but its broader utility remains underexplored due to limited published studies. Key gaps include:
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Functional Characterization: No data exist on the antibody’s ability to inhibit or modulate At1g19565 protein activity.
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Cross-Reactivity: Potential binding to homologous proteins in other plant species (e.g., Arabidopsis lyrata, Brassica napus) is untested.
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Epitope Mapping: The exact binding region (epitope) on the At1g19565 protein is unspecified, limiting insights into antibody specificity.
Product Specs
Q&A
The At1g19565 antibody is a polyclonal reagent raised against recombinant Arabidopsis thaliana protein, primarily used in ELISA and Western blot (WB) applications . Below are structured FAQs addressing key methodological considerations for researchers, categorized by complexity and grounded in experimental design principles.
Advanced Methodological Challenges
How to resolve inconsistent WB signals with At1g19565 antibody?
| Factor | Optimization Strategy |
|---|---|
| Protein Load | Titrate between 10-50 μg/lane |
| Blocking | Compare 5% BSA vs. non-fat milk (BSA reduces lipid-protein interactions) |
| Antibody Dilution | Test 1:500 to 1:2000 in TBST with 1% BSA |
What experimental designs address potential cross-reactivity in non-model plants?
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Perform phylogenetic alignment of At1g19565 homologs.
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Use peptide competition assays: Pre-incubate antibody with 10x molar excess of immunogen peptide .
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Validate with mass spectrometry after immunoprecipitation.
Data Interpretation Scenarios
How to distinguish true signal from background in low-abundance targets?
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Day 1: Chemiluminescent detection with 10-min substrate incubation.
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Day 2: Repeat with fluorescent secondary antibodies (e.g., IRDye 800CW) for quantitative comparison .
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Statistical baseline: Calculate signal-to-noise ratio ≥3:1 across three biological replicates.
When should researchers question antibody performance in time-course experiments?
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If >20% variance in housekeeping protein-normalized data occurs between T0 and T1 timepoints.
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Required controls:
