At1g34300 Antibody
Description
Introduction to AT1G34300 Antibody
AT1G34300 antibody is a research tool targeting the Arabidopsis thaliana gene locus AT1G34300, which encodes a lectin protein kinase family protein. This antibody is primarily used in plant molecular biology to study protein function, post-translational modifications, and signaling pathways .
Protein Localization Studies
The antibody has been used to detect AT1G34300 in:
Stress Response Mechanisms
Key findings from salt stress experiments:
| Treatment | log₂ Fold Change | FDR |
|---|---|---|
| NaCl 24h + SAHA 24h | 5.000 | 2.84E-05 |
| NaCl 2h + SAHA 24h | 5.167 | 2.84E-05 |
Data shows significant upregulation under combined histone deacetylase inhibition and salinity stress .
Functional Insights from Phosphorylation Studies
AT1G34300 exhibits phosphorylation at residues 560-575, critical for kinase activation:
| Study | Phosphorylation Site | Method |
|---|---|---|
| MASCP Gator Analysis | 560–575 | Mass spectrometry |
| Kinase Activity Assay | Ser-562 | Radioactive labeling |
This post-translational modification modulates interactions with auxin-responsive proteins .
Antibody Specificity
Validation parameters:
| Test | Result |
|---|---|
| Western Blot | Single band at ~70 kDa |
| ELISA Cross-Reactivity | <5% with At1G11330 |
| Immunoprecipitation | Recovers intact kinase complex |
Experimental Protocols
Standardized working concentrations:
| Application | Concentration |
|---|---|
| Western Blotting | 1:2,000 |
| Immunofluorescence | 1:500 |
| ELISA | 0.2 µg/mL |
Comparative Analysis with Related Proteins
Functional redundancy in lectin kinases:
| AGI Code | Expression Pattern | Stress Response Role |
|---|---|---|
| AT1G34300 | Root-specific | Salinity adaptation |
| AT1G11330 | Leaf vasculature | Pathogen defense |
| AT3G09270 | Whole plant | Heavy metal detox |
AT1G34300 shows unique spatial expression restricted to root tissues under abiotic stress .
Research Limitations
Current challenges include:
-
Lack of crystal structure data for epitope mapping
-
Limited in vivo functional studies in mutant lines
Future Directions
Priority research areas:
-
CRISPR-Cas9 knockout line characterization
-
Structural analysis of lectin-kinase domain interface
-
High-throughput phosphoproteomics under drought stress
