At1g24060 Antibody

Introduction

The At1g24060 antibody is a specialized immunological reagent designed to detect the Arabidopsis thaliana protein encoded by the At1g24060 gene. This antibody plays a critical role in plant biology research, particularly in studies involving molecular characterization, protein localization, and functional analysis of this uncharacterized protein. Below, we synthesize data from commercial vendors, peer-reviewed studies, and technical specifications to provide a detailed profile of this reagent.

Target Protein: At1g24060

The At1g24060 gene in Arabidopsis thaliana encodes a putative uncharacterized protein with limited functional annotation. Key features include:

• Gene ID: AT1G24060

• Synonyms: T23E23.21, hypothetical protein AT1G24060

• Protein length: Not explicitly stated in available sources, but recombinant versions span ~200–630 amino acids depending on expression systems .

Polyclonal Antibody (Rabbit)

• Host: Rabbit

• Immunogen: Synthetic peptides or recombinant protein derived from At1g24060 .

• Purification: Antigen-affinity chromatography .

• Applications:

  • Western blot (WB): Detects bands at expected molecular weights in Arabidopsis extracts .

  • ELISA: Used for quantitative analysis of At1g24060 in experimental samples .

Recombinant Protein Production

Recombinant At1g24060 proteins are produced in multiple systems for antibody validation :

Functional Studies

• Protein Localization: Identifies subcellular distribution in Arabidopsis tissues .

• Interaction Networks: Used in co-immunoprecipitation (Co-IP) to map binding partners.

Diagnostic and Technical Use

• Western Blot Validation: Detects endogenous At1g24060 in plant extracts .

• ELISA Quantification: Measures protein levels under stress or developmental conditions .

Validation and Limitations

• Specificity Concerns: A study evaluating commercial antibodies for Arabidopsis proteins noted that nonspecific binding is common without rigorous validation . Users must confirm specificity using knockout controls.

• Cross-Reactivity: No data available for cross-species reactivity.

Key Studies

• Expression Analysis: At1g24060 is ubiquitously expressed in Arabidopsis tissues, with elevated levels in roots and leaves under abiotic stress .

• Subcellular Localization: Preliminary data suggest nuclear and cytoplasmic localization .

Technical Challenges

• Antibody Validation: As highlighted in studies on analogous plant antibodies, false-positive signals may arise due to epitope similarity with unrelated proteins .

Purchasing and Customization

• Standard Packages: Pre-made combinations (e.g., N- and C-terminal antibodies) are available for WB applications at ~$899 .

• Custom Development: Vendors like Abmart and Cusabio offer tailored monoclonal antibodies for unique epitopes or functional studies (starting at $599) .

Future Directions

• Functional Characterization: CRISPR/Cas9 knockout models paired with At1g24060 antibodies could elucidate its role in plant development.

• Structural Studies: Cryo-EM or X-ray crystallography using antibody-antigen complexes may reveal 3D protein architecture.