At2g23950 Antibody

Key Product Details

Source: Cusabio product listing .

Research Applications

The At2g23950 antibody is primarily utilized in Western blotting and kinase activity assays. Its applications include:

• Detection of endogenous At2g23950 protein in plant tissue lysates.

• Analysis of phosphorylation states in kinase signaling pathways .

• Functional studies exploring the role of At2g23950 in plant development and stress responses.

In one study, recombinant At2g23950 protein expressed in E. coli failed to undergo autophosphorylation, suggesting improper folding or the need for specific cofactors . This finding highlights the antibody’s utility in validating protein expression and stability in experimental systems.

Kinase Activity and Phosphorylation

• At2g23950 is an RD-type kinase implicated in signal transduction. Unlike its close homolog BRI1 (brassinosteroid-insensitive 1), which undergoes tyrosine phosphorylation, recombinant At2g23950 showed no autophosphorylation activity under standard conditions .

• The antibody was critical in confirming the absence of phosphotyrosine residues in At2g23950, distinguishing its biochemical behavior from other RLKs .

Technical Performance

• Sensitivity: The antibody detects At2g23950 at concentrations as low as 1 µg/mL in Western blots .

• Specificity: No cross-reactivity with unrelated Arabidopsis proteins has been reported .

Broader Implications

The At2g23950 antibody facilitates research into plant receptor kinases, which are essential for understanding:

• Cell surface signaling mechanisms in response to environmental stimuli.

• Evolutionary divergence among RD kinase family members.

• Protein folding requirements for kinase activation.

Limitations and Future Directions

• Unresolved folding issues: The lack of autophosphorylation in recombinant At2g23950 warrants further structural studies .

• Expanded applications: Testing the antibody in immunohistochemistry or in planta localization assays could enhance its utility.