Customize At4g26485 Antibody

Description

Understanding the Compound Identifier

The identifier "At4g26485" follows the naming convention for Arabidopsis thaliana genes (e.g., At for Arabidopsis thaliana; "4g" denotes chromosome 4, and "26485" is a unique gene number). This suggests the compound may relate to a gene or protein in Arabidopsis rather than a characterized antibody.

No search results mention antibodies specific to Arabidopsis proteins, plant immunology, or gene-encoded antibodies. The provided sources focus on human/animal antibodies (e.g., Alzheimer’s disease, myasthenia gravis, malaria, and migraine therapies) .

Possible Explanations for the Absence of Data

Reason	Details
Misinterpretation of Nomenclature	"At4g26485" may refer to a gene rather than an antibody. Antibodies are typically named based on their target antigen or therapeutic application (e.g., "anti-EGFR," "anti-PACAP") .
Niche or Emerging Research	Antibodies targeting plant proteins are rare in clinical or biomedical research. Most studies focus on human pathogens or diseases .
Data Limitations	The provided sources exclude agricultural or plant biology literature, which might contain relevant studies on Arabidopsis antibodies.

Guidance for Further Research

To investigate "At4g26485 Antibody," consider the following steps:

Verify the Target
- Confirm whether "At4g26485" refers to a gene, protein, or hypothetical antibody construct.
- Cross-reference with Arabidopsis genome databases (e.g., TAIR, Araport) to identify the gene’s function or associated proteins.
Explore Plant Immunology Resources
- Search specialized journals (e.g., Plant Cell, Molecular Plant) for studies on plant-specific antibodies.
- Investigate agricultural biotechnology applications, such as pest-resistant crops or plant disease diagnostics.
Check Antibody Databases
- Use repositories like cAb-Rep or commercial antibody catalogs (e.g., Absolute Antibody ) to identify antibodies targeting plant proteins.

Example of Antibody Nomenclature and Structure

While no data exists for "At4g26485 Antibody," the table below illustrates how antibodies are typically characterized:

Property	Description	Example
Target	Specific antigen or protein	PACAP (migraine therapy) , LRP4 (myasthenia gravis)
Isotype	Antibody class (IgG, IgM, etc.)	IgG1 (common therapeutic isotype)
Format	Monoclonal, chimeric, or engineered	Chimeric antibodies (humanized variable regions)
Function	Neutralizing, blocking, or agonistic	Neutralizing tau tangles (Alzheimer’s)

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Composition: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

At4g26485 antibody; M3E9.90 antibody; Uncharacterized protein At4g26485 antibody

Target Names

At4g26485

Uniprot No.

P0C8L4

Q&A

Advanced Technical Challenges

How to resolve contradictory ChIP-seq and immunolocalization data?

Scenario: Antibody shows nuclear staining but fails ChIP-seq validation
Troubleshooting protocol:
- Confirm epitope accessibility via antigen retrieval optimization
- Test cross-reactivity with in vitro translated homologs (e.g., At4g26480/90)
- Validate using CRISPR-edited lines with disrupted antibody-binding domains

Optimizing co-IP for low-abundance transcription factors

Key parameters:
- Crosslinker choice: Compare formaldehyde (reversible) vs. DSP (thiol-cleavable)
Quantify pull-down efficiency via spike-in controls (e.g., 15N-labeled recombinant protein)

Data Interpretation Guidance

Addressing variable binding affinity measurements

Case study: IL-4Rα antibodies showed 100-fold affinity differences between monovalent Fab (2.36 nM) and bivalent IgG (9.16 pM)
Solution matrix:

Experimental Need	Recommended Format	Rationale
Structural studies	Fab fragments	Avoid avidity effects
Functional blocking	Full IgG	Leverage bivalent binding

When to question commercial antibody claims

Red flags from :
- Consistent detection of 100 kDa bands across species without evolutionary conservation
- Failure to replicate IP-MS results with multiple lots

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Source

Quantity&Size*

Product Name

Message

At4g26485 Antibody