YAL063C-A Antibody

Introduction

The YAL063C-A antibody is a high-specificity reagent targeting the YAL063C-A protein, a poorly characterized gene product in Saccharomyces cerevisiae (budding yeast). This antibody is employed in molecular biology research to study protein localization, expression, and interactions. Its development and application are critical for elucidating the function of YAL063C-A, which has been implicated in cellular processes such as cell wall biogenesis and flocculation .

Research Applications

The antibody is primarily used in:

1. Protein localization studies: Via fluorescence microscopy or immunofluorescence assays (IFA) to track YAL063C-A in yeast cells .

2. Western blotting: For quantifying protein levels during growth phases or under stress conditions .

3. Co-immunoprecipitation (Co-IP): To identify interacting partners of YAL063C-A, such as FLO1 and FLO5 proteins .

4.1. Role in Cell Wall Biogenesis

YAL063C-A is orthologous to FLO1 homologues (e.g., YKR102w, YHR211w), which encode mannoproteins linked to mannose-6-phosphate residues in the cell wall . Studies using the antibody revealed:

• FLO1-dependent flocculation: YAL063C-A is expressed during pseudohyphal growth and interacts with FLO11 promoters .

• Cell wall integrity: Deletion of YAL063C-A disrupts CCW12/CCW13/CCW14 glycoprotein complexes, impairing mating efficiency and cell wall stability .

4.2. Genetic Interactions

The antibody has been used to demonstrate epistatic relationships between YAL063C-A and IME1/IME4 transcription factors, which regulate meiotic gene expression .

4.3. Subcellular Localization

IFA experiments show YAL063C-A localizes to the cell surface and bud neck during mitosis, suggesting a role in polarized growth .

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