YBL077W Antibody
Description
Gene and Protein Function
The YBL077W gene encodes a conserved protein of unknown function in S. cerevisiae. Proteomic studies have identified it as part of the yeast proteome, with evidence supporting its essentiality:
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Essentiality: YBL077W is classified as an essential ORF (open reading frame), meaning its deletion results in nonviability under standard laboratory conditions .
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Expression Patterns: Microarray data from Gasser Lab indicate moderate expression levels (e.g., 0.5668 for YBL077W in protein kinase domain analysis) .
Antibody Validation and Applications
The YBL077W antibody has been validated in yeast proteome studies, particularly for detecting glycoproteins. Key findings include:
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Glycoprotein Detection: A polyclonal antibody recognizing yeast glycans (used in MORF library screening) identified YBL077W as a glycoprotein candidate. Western blot analysis confirmed its glycosylation via gel-shift assays .
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Protein Interactions: The antibody has been used to study interactions with other yeast proteins, including YAR020C (membrane protein) and YAR027W (FUN55 homolog) .
Research Implications
The YBL077W antibody serves as a critical tool for studying yeast glycoproteins and essential genes. Its applications extend to:
Product Specs
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
