YBR139W Antibody
Description
Biological Role of YBR139W
YBR139W encodes a 57.6 kDa soluble vacuolar protease with a serine carboxypeptidase catalytic triad (Ser219, Asp415, His474) . Key functional attributes include:
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Autophagy: Mediates terminal proteolytic steps during autophagic body degradation .
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Zymogen Activation: Processes precursor forms of vacuolar hydrolases like prApe1 and intPrb1 .
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Functional Redundancy: Shares overlapping roles with Prc1 (carboxypeptidase Y) in protein processing under starvation conditions .
Antibody Applications in Research
The YBR139W antibody is critical for:
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Localization Studies: Tracking vacuolar delivery via GFP-tagged fusion proteins (e.g., Ybr139w-GFP cleavage assays) .
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Functional Analysis: Assessing enzymatic activity through mutagenesis of catalytic residues (e.g., Ser219Ala abolishes activity) .
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Protein Interaction Studies: Examining dependencies on trafficking factors like Vps10 for vacuolar delivery .
Key Research Findings
Protein Characteristics
| Property | Value | Source |
|---|---|---|
| Molecular Weight | 57.6 kDa | |
| Isoelectric Point (pI) | 4.94 | |
| Half-Life | 8.2 hours | |
| Cellular Abundance | ~4,606 molecules/cell |
Experimental Validation
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
