SPCC584.15c Antibody

Gene and Protein Overview

• Gene ID: SPCC584.15c (also referred to as aly3 in functional studies).

• Protein Function: Encodes an α-arrestin-like protein involved in:

  • Ubiquitin-dependent endocytosis: Mediates the sorting of membrane-associated proteins to vacuoles via ESCRT machinery .

  • TORC2 signaling regulation: Inhibits endocytosis under nitrogen-rich conditions by modulating Ght5-GFP ubiquitylation .

  • Cell wall synthesis: Plays a role in the covalent linkage of GPI-anchored proteins to β-1,6-glucan, critical for cell wall integrity .

Antibody Development and Applications

The SPCC584.15c antibody is likely used to study the protein’s localization and function in yeast models. Key experimental uses include:

• Immunoprecipitation: To isolate the Aly3 protein and its ubiquitylated forms .

• Western blotting: Detects Aly3 expression levels under different growth conditions (e.g., nitrogen starvation) .

• Immunolocalization: Visualizes Aly3 distribution in subcellular compartments (e.g., vacuoles, cell membrane) .

TORC2 Signaling and Endocytosis

• Key Discovery: Aly3-dependent ubiquitylation of Ght5 (a transmembrane protein) is inhibited by TORC2-Gad8 signaling under nitrogen-rich conditions .

• Mechanism:

  • Nitrogen replenishment → TORC2 activation → Inhibition of Aly3-mediated ubiquitylation → Reduced Ght5 endocytosis .

  • Nitrogen deprivation → TORC2 inactivation → Increased Aly3-dependent ubiquitylation → Enhanced Ght5 degradation .

Cell Wall Integrity

• Role in β-1,6-Glucan Synthesis: SPCC584.15c is essential for proper β-1,6-glucan formation, a key component of the yeast cell wall .

• Knockdown Effects: Depletion of SPCC584.15c leads to:

  • Aberrant septum formation and cell wall defects .

  • Accumulation of β-1,3-glucan in the septum, disrupting normal cell division .

Future Research Directions

• Therapeutic Potential: Investigating SPCC584.15c homologs in higher eukaryotes for roles in human diseases (e.g., fungal infections, lysosomal disorders).

• Antibody Engineering: Developing antibodies with enhanced specificity for Aly3 to study its conservation across species.