SPCC1795.12c Antibody

Protein Expression and Detection

The antibody has been validated for detecting SPCC1795.12c via Western blot and ELISA. In fission yeast lysates, it identifies a protein band at ~13 kDa, consistent with its predicted molecular weight . Its use in immunofluorescence studies suggests localization to membrane-bound organelles, though functional studies remain limited .

Functional Context

While SPCC1795.12c is annotated as a sequence orphan, systematic deletion studies of transcription factors (TFs) in S. pombe have included analyses of related uncharacterized genes. For example:

• Phenotypic Screening: TF deletion strains (e.g., SPBC56F2.05Δ) were screened for sensitivity to stressors like tunicamycin, revealing indirect connections to cell wall integrity pathways .

• Microarray Profiling: Genome-wide expression analyses of TF deletion strains have identified co-regulated genes, though SPCC1795.12c itself has not been directly linked to specific pathways .

1. Technical Validation and Quality Control

• Purity: The antibody is affinity-purified, ensuring minimal cross-reactivity with non-target proteins .

• Storage: Stable at -20°C or -80°C; repeated freeze-thaw cycles are discouraged .

• Certification: Manufactured in an ISO 9001:2015-certified laboratory .

1. Limitations and Future Directions

• Uncharacterized Function: The biological role of SPCC1795.12c remains unknown, necessitating further studies such as knockout strain phenotyping or interactome analyses.

• Species Specificity: Reactivity is confined to S. pombe, limiting its utility in cross-species comparisons .