SPCC1393.09c Antibody

Biological Context of SPCC1393.09c

While the precise function of SPCC1393.09c in S. pombe is not explicitly detailed in the provided sources, its genomic context and related studies suggest potential roles:

• Genomic Association: SPCC1393.09c is annotated as a hypothetical protein with no direct functional characterization in the literature reviewed.

• Expression Data: Microarray analyses of S. pombe acetyltransferase mutants (e.g., Δgcn5, Δmst2) did not identify SPCC1393.09c as differentially expressed, implying stable expression under tested conditions .

Key Features

• Specificity: The antibody is affinity-purified, ensuring minimal cross-reactivity with non-target proteins in S. pombe lysates .

• Technical Applications:

  • Western Blotting: Likely validated for detecting SPCC1393.09c in whole-cell extracts (typical dilution range: 1:500–1:2000).

  • Immunoprecipitation: Potential utility in isolating SPCC1393.09c complexes for interactome studies .

Limitations

• No peer-reviewed studies directly utilizing this antibody were identified, necessitating independent validation for novel applications.

Comparative Analysis with Related Antibodies

Antibodies targeting other S. pombe proteins (e.g., Slx8, Ufd1, or TOR pathway regulators) highlight common workflows for fission yeast research:

Future Research Directions

• Functional Studies: CRISPR-mediated deletion or overexpression of SPCC1393.09c, paired with phenotypic screening (e.g., Phloxine B viability assays ).

• Interactome Profiling: Use of the SPCC1393.09c Antibody in immunoprecipitation-mass spectrometry (IP-MS) to map binding partners.

• Subcellular Localization: Immunofluorescence to determine spatial expression patterns under stress conditions.