YDR210W Antibody

Introduction to YDR210W and Its Antibody

YDR210W is a yeast gene encoding a cysteine-rich, tail-anchored plasma membrane protein containing a conserved cysteine-rich palmitoylated domain (CYSPD) . The YDR210W antibody targets this protein, facilitating its detection in experimental workflows such as immunoprecipitation, Western blotting, and fluorescence microscopy .

Functional Insights

• Role in Membrane Dynamics: Involved in endocytosis and recycling, mediated by interactions with clathrin adaptors (e.g., Sla1Δ and Ric1Δ) .

• Stress Response: Orthologs in other eukaryotes are associated with metal detoxification and pathogen resistance .

Production and Validation of YDR210W Antibodies

YDR210W antibodies are typically produced as polyclonal reagents using recombinant protein immunogens . Key validation steps include:

• Specificity Testing: Western blotting against yeast lysates to confirm target band size (~12–15 kDa) .

• Application Validation: Demonstrated utility in immunofluorescence (IF) for subcellular localization studies .

Research Applications

YDR210W antibodies have been employed in:

1. Protein-Protein Interaction Studies: Identification of interactors via immunoprecipitation coupled with mass spectrometry (IP-MS) .

2. Localization Mapping: Visualization of polarized PM distribution using GFP-tagged constructs .

3. Functional Knockdown Assays: Assessing phenotypes in yeast strains with disrupted YDR210W expression .

Challenges and Considerations

• Specificity: Cross-reactivity with homologous yeast proteins (e.g., YDL012C) requires rigorous validation .

• Stability: Protein degradation in akr1Δ or erf2Δ strains complicates detection .

• Localization Dynamics: Polarized distribution necessitates high-resolution microscopy for accurate interpretation .

Future Directions

Ongoing studies aim to:

1. Elucidate YDR210W’s role in metal homeostasis and stress adaptation.

2. Explore therapeutic potential via orthologs in pathogenic fungi .

3. Develop monoclonal YDR210W antibodies for enhanced specificity .