SPAC9G1.14 Antibody

Introduction to SPAC9G1.14 Antibody

SPAC9G1.14 Antibody is a polyclonal immunoglobulin raised against the putative uncharacterized protein SPAC9G1.14 from Schizosaccharomyces pombe, commonly known as fission yeast. This antibody serves as an important research tool for the detection, localization, and functional analysis of its target protein. The antibody is specifically designed to recognize epitopes on the SPAC9G1.14 protein with high specificity and affinity, enabling researchers to investigate this protein's expression patterns, interactions, and potential roles within the cellular machinery of S. pombe .

Polyclonal antibodies like SPAC9G1.14 Antibody contain a heterogeneous mixture of immunoglobulins that recognize multiple epitopes on their target antigen. This characteristic often provides robust detection capabilities across various experimental conditions and applications. The production of such antibodies typically involves immunizing host animals with purified antigen, followed by isolation and purification of the resulting antibodies from the host's serum .

The development of specific antibodies against S. pombe proteins, including SPAC9G1.14, represents an important advancement in yeast research tools. S. pombe serves as a valuable model organism in molecular and cellular biology, particularly for studying cell cycle regulation, chromosome dynamics, and other fundamental cellular processes. Antibodies targeting specific proteins within this organism enable detailed investigations that contribute to our understanding of conserved biological mechanisms with potential relevance to human health and disease .

Antibody Structure and Properties

SPAC9G1.14 Antibody is classified as an IgG isotype polyclonal antibody raised in rabbits. The antibody is supplied in liquid form, specifically formulated with a storage buffer containing 0.03% Proclin 300 as a preservative, 50% glycerol, and 0.01M PBS at pH 7.4. This formulation ensures stability and longevity of the antibody when stored appropriately .

As a polyclonal IgG antibody, SPAC9G1.14 exhibits the characteristic Y-shaped structure common to immunoglobulins, consisting of two heavy chains and two light chains connected by disulfide bonds. The antigen-binding sites, located at the tips of the Y structure, contain variable regions that specifically recognize and bind to epitopes on the SPAC9G1.14 protein . The polyclonal nature of this antibody means it contains a mixture of immunoglobulins that can recognize different epitopes on the target protein, potentially enhancing detection sensitivity across various experimental conditions.

Manufacturing Process

SPAC9G1.14 Antibody is produced through a systematic immunization protocol using rabbits as the host organism. The immunization process involves introducing the recombinant SPAC9G1.14 protein to stimulate an immune response. Following immunization, the antibodies are harvested from rabbit serum and subsequently purified using antigen affinity chromatography to isolate antibodies specifically targeting the SPAC9G1.14 protein .

This purification method ensures high specificity by selectively capturing antibodies that bind to the target protein while removing other serum components. The resulting purified antibody preparation maintains strong binding affinity for the target antigen while minimizing non-specific interactions that could compromise experimental results .

Technical Parameters

A comprehensive overview of the technical specifications for SPAC9G1.14 Antibody is presented in Table 1.

Validated Experimental Applications

SPAC9G1.14 Antibody has been validated for use in specific laboratory techniques, primarily Enzyme-Linked Immunosorbent Assay (ELISA) and Western Blot (WB) applications. These validated applications make the antibody valuable for researchers seeking to detect and quantify SPAC9G1.14 protein expression in various experimental contexts .

In ELISA applications, the antibody can be used to detect and quantify SPAC9G1.14 protein in solution. This technique allows for sensitive detection of the target protein and can be particularly useful for screening samples or quantifying protein levels under different experimental conditions. The polyclonal nature of the antibody may provide robust detection capability across different ELISA formats, including direct, indirect, sandwich, or competitive assays .

For Western Blot applications, SPAC9G1.14 Antibody enables the detection of the target protein following separation by gel electrophoresis. This application provides information about protein expression levels, molecular weight, and potential post-translational modifications. The specificity of the antibody ensures accurate identification of the target protein among complex protein mixtures .

Potential Research Applications

While specific research findings using SPAC9G1.14 Antibody are not extensively documented in the available literature, the antibody's properties suggest potential utility in various research contexts focused on S. pombe biology. Possible applications include:

1. Expression profiling of SPAC9G1.14 protein under different growth conditions or in response to various stressors

2. Investigation of protein-protein interactions involving SPAC9G1.14 through co-immunoprecipitation studies

3. Localization studies using immunofluorescence techniques to determine the subcellular distribution of SPAC9G1.14

4. Functional characterization of SPAC9G1.14 through knockdown or knockout studies, using the antibody to confirm altered expression levels

The antibody's specificity for S. pombe makes it particularly valuable for researchers using this model organism to study fundamental cellular processes. As a research tool, it contributes to the broader toolkit available for investigating protein function and cellular biology in fission yeast .

Handling Recommendations

When working with SPAC9G1.14 Antibody, several handling practices can help maximize experimental success:

1. Centrifuge the vial briefly before opening to ensure all liquid is collected at the bottom of the tube

2. Avoid repeated freezing and thawing by preparing single-use aliquots

3. Keep the antibody on ice when in use during laboratory procedures

4. Dilute the antibody in appropriate buffers according to the specific experimental protocol

5. Include appropriate controls in all experiments to validate antibody performance

These handling recommendations are consistent with general best practices for antibody use in laboratory settings and help ensure optimal performance of the SPAC9G1.14 Antibody in experimental applications .

Comparison with Related Antibodies

In the broader context of antibody tools for S. pombe research, SPAC9G1.14 Antibody represents a specialized reagent targeting a specific uncharacterized protein. While comprehensive comparative data with other antibodies is limited in the available literature, it is worth noting that specific antibodies against uncharacterized proteins like SPAC9G1.14 serve an important role in functional genomics studies aimed at elucidating the roles of previously unstudied gene products .

For comparative purposes, Table 2 presents a general comparison between SPAC9G1.14 Antibody and another S. pombe-specific antibody mentioned in the search results.

Table 2: Comparative Analysis of S. pombe-Specific Antibodies

While both antibodies target S. pombe proteins and share similar technical specifications, they recognize different proteins with potentially distinct cellular functions .

Research Context in Antibody Development

The development of SPAC9G1.14 Antibody represents a broader trend in molecular biology research tools where antibodies against uncharacterized or putative proteins are generated to facilitate functional studies. In the context of model organism research, such antibodies play a crucial role in moving from genomic sequence information to functional characterization of gene products .

The production of polyclonal antibodies against recombinant proteins, as exemplified by SPAC9G1.14 Antibody, demonstrates a standard approach in research tool development. This approach offers advantages in terms of recognizing multiple epitopes on the target protein, potentially enhancing detection sensitivity across various experimental conditions .