SPAC22A12.17c Antibody

Gene Overview: SPAC22A12.17c

• Organism: Schizosaccharomyces pombe (fission yeast).

• Function: Encodes a protein involved in stress responses, particularly under KCl-induced stress.

• Regulation:

  • Upregulated >19-fold in wild-type cells treated with KCl compared to untreated controls .

  • Derepressed >2-fold in Δatf1 (lacking the transcription factor Atf1) and Δpcr1 (lacking the transcription factor Pcr1) mutants under untreated conditions .

  • Requires the transcription factor Pcr1 for optimal expression under KCl stress .

Stress-Response Role

The gene is part of a subset of KCl-dependent genes that are derepressed in Δatf1 and Δpcr1 mutants, suggesting its expression is normally repressed by these transcription factors under basal conditions . Its upregulation in KCl-treated wild-type cells highlights its role in mitigating cellular stress, potentially through osmoregulation or oxidative stress pathways.

Lack of Antibody-Specific Data

No commercial or experimental antibodies targeting SPAC22A12.17c are mentioned in the provided sources. Antibody development for yeast proteins typically involves challenges such as low cross-reactivity with mammalian systems and limited commercial demand. Researchers interested in studying this gene would likely need to generate custom antibodies or use alternative methods (e.g., GFP tagging or qRT-PCR) for analysis.

Key Experimental Findings

• Untreated Δatf1 cells: SPAC22A12.17c expression is derepressed >3-fold compared to wild-type .

• KCl-treated Δpcr1 cells: Expression remains elevated (2.16-fold), indicating partial dependency on Pcr1 for stress-induced activation .

Broader Context

The study underscores the complex interplay between transcription factors Atf1 and Pcr1 in regulating stress-responsive genes. SPAC22A12.17c’s differential expression across genetic backgrounds suggests it may participate in compensatory pathways to maintain cellular homeostasis under stress .