YHR032W-A Antibody

Basic Identification and Target Information

YHR032W-A antibody (Product Code: CSB-PA313635XA01SVG) is a monoclonal antibody developed for detecting the YHR032W-A protein, encoded by the YHR032W-A gene in yeast. This protein belongs to the MatE family of transporters and shares homology with multidrug/toxin extrusion (MATE) transporters .

Target Protein Attributes

• Molecular Weight: ~50 kDa (predicted based on amino acid sequence) .

• Domain Structure: Contains 12 transmembrane helices characteristic of MATE transporters .

• Function: Implicated in ethionine resistance and S-adenosyl-l-methionine (AdoMet) homeostasis .

Antibody Architecture

• Composed of two heavy chains (~50 kDa) and two light chains (~25 kDa) .

• Utilizes a paratope (antigen-binding site) formed by variable regions of heavy and light chains for target specificity .

Functional Studies

• Overexpression of YHR032W in yeast (strain W303-1A) failed to confer ethionine resistance, unlike its gain-of-function mutant SSG1-1 .

• A frameshift mutation in SSG1-1 (a YHR032W allele) results in a C-terminal extension (+36 amino acids), enhancing transporter activity .

Gene Activation Data

YHR032W-A exhibits a 3.5-fold activation under stress conditions linked to sphingolipid metabolism and oxidative response :

Applications in Research

• Western Blot: Validated for detecting YHR032W-A in yeast lysates, showing specificity in knockout vs. wild-type controls .

• Immunoprecipitation: Used to study protein-protein interactions involving AdoMet synthesis pathways .

• Functional Genomics: Supports investigations into yeast stress responses and drug-resistance mechanisms .

Validation and Quality Control

• Specificity: Verified using YHR032W-A knockout yeast strains to eliminate cross-reactivity .

• Performance Metrics:

  • Western Blot: Band specificity confirmed in wild-type lysates .

  • Immunofluorescence: Limited utility due to poor selectivity in non-blot applications .

Comparative Analysis with Antibody Databases

• Listed in AbDb (Antibody Structure Database) under Saccharomyces cerevisiae entries, annotated with Kabat/Chothia numbering schemes .

• Metadata accessible via PLAbDab (Patent and Literature Antibody Database), highlighting its sequence redundancy and structural classification .

Future Directions

• Therapeutic Potential: Explore roles in antifungal drug resistance.

• Structural Biology: Cryo-EM studies to resolve its transporter mechanism.