YHR122W Antibody

YHR122W Antibody: Overview and Generation

The YHR122W antibody targets the CIA2 protein (UniProt ID: P40089), a 22 kDa polypeptide containing a DUF59 domain essential for Fe/S cluster transfer . Antibodies against CIA2 are typically generated using recombinant protein fragments or synthetic peptides as immunogens. For example, studies have employed murine myc-tagged CIA2A (a human ortholog) for antibody validation . These antibodies are often monoclonal or polyclonal, with specificity confirmed via immunoblotting and RNA interference (RNAi)-mediated depletion assays .

Applications of YHR122W Antibody in Research

The antibody is widely used to investigate CIA2’s role in Fe/S cluster biogenesis and its interactions with other CIA components. Key applications include:

Role in Fe/S Protein Maturation

• CIA2 forms a targeting complex with CIA1 and MET18 to direct Fe/S clusters to apoproteins like dihydropyrimidine dehydrogenase (DPYD) and iron regulatory protein 1 (IRP1) .

• Depletion of CIA2B (human FAM96B) reduces DPYD activity by 85% and destabilizes IRP1, confirming its role in cluster transfer .

Functional Specificity

• CIA2A (FAM96A) and CIA2B (FAM96B) exhibit non-redundant roles: CIA2B is essential for DPYD maturation, while CIA2A is dispensable .

• CIA2B deficiency enhances IRP1’s RNA-binding activity, suggesting regulatory feedback in iron metabolism .

Role in Antifungal Drug Discovery

The YHR122W gene is a validated antifungal target due to its essentiality for yeast survival . The CIA2 antibody facilitates:

• Target Validation: Confirming CIA2’s indispensability via RNAi and growth assays .

• Compound Screening: Identifying inhibitors using ELISA, RIA, or yeast viability assays .

• Mechanistic Studies: Resolving structural interactions of CIA2 with drug candidates .