SPCC757.15 Antibody

Target Characterization

Gene Name: SPCC757.15
UniProt ID: C6Y4C7
Protein Class: Uncharacterized membrane protein
Subcellular Localization: Single-pass membrane protein (predicted)
Species Reactivity: Schizosaccharomyces pombe (strain 972/24843)

Functional Context:
While the precise role of SPCC757.15 is not fully elucidated, studies on fission yeast cell wall dynamics suggest it may participate in membrane-related processes. For example, depletion of homologous proteins in S. pombe has been linked to cell wall remodeling and glucanase activity modulation .

Research Applications and Validation

Key Applications:

• Western Blot: Validated for detecting SPCC757.15 in S. pombe lysates .

• ELISA: Titer guaranteed at 1:64,000 .

Validation Challenges:

• Lack of knockout (KO) cell line data for specificity confirmation (common issue for uncharacterized proteins).

• Reliance on sequence homology for epitope prediction due to limited structural data .

Related Research Findings

While direct studies on SPCC757.15 are scarce, insights from analogous proteins in fission yeast highlight potential roles:

Cell Wall Dynamics:

• SPCC757.15 depletion may trigger glucanase upregulation, akin to Sup11p in S. pombe, which induces β-1,3-glucan accumulation at septa .

• Transcriptome analyses suggest coordinated regulation with other cell wall enzymes .

Technical Notes:

• Antibody performance in WB and ELISA is contingent on antigen accessibility, given the protein’s membrane localization .

• No peer-reviewed studies explicitly confirm SPCC757.15’s function, underscoring the need for further characterization.

Critical Considerations

• Specificity: Cross-reactivity with unrelated proteins has not been ruled out in published data .

• Reproducibility: Adherence to standardized protocols (e.g., buffer conditions, blocking agents) is critical for reliable results .

• Antibody Characterization Crisis: ~50% of commercial antibodies lack adequate validation, emphasizing the need for independent verification .

Future Directions

• Structural analysis (e.g., cryo-EM) to map epitope binding sites.

• Functional studies using CRISPR/Cas9 KO strains to delineate SPCC757.15’s role in S. pombe.

• Collaboration with repositories like the Human Protein Atlas to expand validation datasets .