SPAC607.06c Antibody

Introduction to SPAC607.06c Antibody

SPAC607.06c Antibody is a polyclonal antibody specifically developed to recognize and bind to the SPAC607.06c protein in Schizosaccharomyces pombe (strain 972 / ATCC 24843), commonly known as fission yeast. The antibody targets a protein identified by the UniProt accession number Q9US12, indicating its registration in established protein databases that catalog and organize protein information for scientific research purposes . This antibody represents an important tool for researchers studying fission yeast cellular processes and protein functions.

The target protein, SPAC607.06c, belongs to the proteome of Schizosaccharomyces pombe, an organism that serves as a valuable model system in molecular and cellular biology research. Fission yeast has gained significant importance in the scientific community due to its utility in studying fundamental cellular processes including cell division, DNA replication, and gene expression. The availability of specific antibodies like SPAC607.06c Antibody enables researchers to investigate protein localization, expression levels, and interactions within this model organism .

Polyclonal antibodies such as SPAC607.06c Antibody offer certain advantages in research applications, particularly their ability to recognize multiple epitopes on the target antigen. This characteristic can enhance detection sensitivity in various experimental conditions, making them valuable for initial characterization studies of proteins like SPAC607.06c .

Production and Purification Methods

The production of SPAC607.06c Antibody follows established immunological techniques for polyclonal antibody generation. The process begins with the preparation of the immunogen, which consists of recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) SPAC607.06c protein. This recombinant protein serves as the antigen that stimulates the immune response in the host animal .

Rabbits are utilized as the host species for antibody production, likely chosen for their robust immune response and the substantial volume of antiserum that can be collected. The immunization protocol typically involves multiple injections of the immunogen to elicit a strong immune response, resulting in the production of antibodies against various epitopes of the SPAC607.06c protein .

Following the immunization period and collection of antiserum, the SPAC607.06c Antibody undergoes an antigen affinity purification process. This critical purification method involves passing the collected serum through a column containing immobilized SPAC607.06c protein. This step ensures that only antibodies specifically binding to the target protein are retained, while non-specific antibodies and other serum components are removed. The result is a purified antibody preparation with enhanced specificity toward the SPAC607.06c protein .

The purified antibody is then formulated in a storage buffer containing 50% glycerol, which acts as a cryoprotectant, 0.01M PBS at pH 7.4 to maintain physiological pH, and 0.03% Proclin 300 as a preservative to prevent microbial growth. This formulation is designed to maximize stability during storage and minimize freeze-thaw damage .

Applications and Research Uses

SPAC607.06c Antibody has been validated for specific research applications, primarily ELISA (Enzyme-Linked Immunosorbent Assay) and Western Blotting (WB). These techniques are fundamental in protein research, allowing for the detection, quantification, and characterization of target proteins in complex biological samples .

In Western Blotting applications, SPAC607.06c Antibody can be used to detect the presence and determine the molecular weight of the SPAC607.06c protein in cell or tissue lysates from Schizosaccharomyces pombe. This application is particularly valuable for studying protein expression levels under various experimental conditions or in different genetic backgrounds. The antibody's specificity ensures accurate identification of the target protein among the numerous proteins present in complex biological samples .

For ELISA applications, the antibody allows for quantitative measurement of SPAC607.06c protein levels with high sensitivity. This technique can be especially useful in experiments requiring precise quantification of protein expression or in high-throughput screening approaches .

It is important to note that SPAC607.06c Antibody is explicitly designated "For Research Use Only" and is not intended for diagnostic or therapeutic applications. This designation reflects regulatory requirements and indicates that the antibody has not been validated or approved for clinical applications. Researchers should adhere to this limitation when planning their experimental protocols .

Potential research applications may include studies of protein-protein interactions, protein localization using immunofluorescence techniques, and investigations of protein function through immunoprecipitation, although validation for these applications would be required prior to use .