YNR034W-A Antibody

Role in TORC1 Signaling

• Rapamycin Sensitivity: Unlike Ego2 (a critical TORC1 regulator), Ego4 deletion does not confer rapamycin hypersensitivity, nor does it reduce TORC1 activity (measured by Sch9 phosphorylation) .

• Protein Interactions: No two-hybrid interactions detected between Ego4 and Ego1/Ego2/Ego3 .

Expression and Evolution

• Expression Data: YNR034W-A exhibits condition-dependent expression, with upregulation in nutrient-stressed conditions (e.g., nitrogen depletion) .

• Paralog Divergence: Ego4 lacks functional redundancy with Ego2, suggesting neofunctionalization post-duplication .

Applications in Research

• TORC1 Pathway Studies: Used to distinguish Ego4 from Ego2/Ego3 in functional assays .

• Protein Interaction Mapping: Applied in co-immunoprecipitation (Co-IP) to confirm lack of Ego4-TORC1 complex formation .

• Yeast Genetics: Employed in studies of whole-genome duplication (WGD) paralogs to explore functional divergence .

Key Research Publications

1. SGD Database Entry1: Confirms YNR034W-A as a paralog with no TORC1 involvement.

2. TORC1 Complex Studies38: Demonstrates Ego4’s independence from TORC1 signaling.

3. Cusabio Catalog6: Details antibody specifications for experimental use.