TY1B-NL1 Antibody
Description
Applications in Research
TY1B-NL1 antibody has been utilized to:
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Visualize VLPs: Detect Ty1 particle formation in yeast cells via immunofluorescence .
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Study Retrotransposition: Monitor Ty1 integration events in genomic stability assays.
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Characterize Host Factors: Identify proteins interacting with Ty1 elements during replication .
Table 1: Key Antibody Properties
| Property | Detail | Source |
|---|---|---|
| Target Antigen | TY1B-NL1 Gag-Pol polyprotein (Capsid) | UniProtKB |
| Host Species | Mouse | Cusabio |
| Clonality | Monoclonal | Cusabio |
| Applications | WB, IF, IP | Cusabio |
Table 2: Cross-Reactivity
| Species | Reactivity | Notes |
|---|---|---|
| S. cerevisiae | Positive | Confirmed via Western blot |
| Human | Negative | No homology detected |
Research Findings
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VLP Assembly: TY1B-NL1 antibody confirmed CA’s role in forming VLPs, which encapsulate Ty1 RNA and replication enzymes .
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Host Restriction Factors: Studies using this antibody identified S. cerevisiae proteins that inhibit Ty1 integration, linking retrotransposons to genome defense mechanisms .
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Evolutionary Insights: Comparative analyses revealed structural similarities between Ty1 CA and retroviral capsids, suggesting conserved replication strategies .
Limitations and Future Directions
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Specificity: Limited to yeast models; no cross-reactivity in mammalian systems.
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Functional Studies: Further work is needed to map conformational epitopes and optimize the antibody for quantitative assays (e.g., ELISA).
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
Aspartyl protease (PR) mediates the proteolytic cleavages of the Gag and Gag-Pol polyproteins after assembly of the VLP.
Reverse transcriptase/ribonuclease H (RT) is a multifunctional enzyme that catalyzes the conversion of the retro-elements RNA genome into dsDNA within the VLP. The enzyme displays DNA polymerase activity that can copy either DNA or RNA templates and ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes during plus-strand synthesis and hydrolyzes RNA primers. The conversion leads to a linear dsDNA copy of the retrotransposon that includes long terminal repeats (LTRs) at both ends.
Integrase (IN) targets the VLP to the nucleus, where a subparticle preintegration complex (PIC) containing at least integrase and the newly synthesized dsDNA copy of the retrotransposon must transit the nuclear membrane. Once in the nucleus, integrase performs the integration of the dsDNA into the host genome.
KEGG: sce:YNL284C-B
STRING: 4932.YNL284C-B
