YOR114W Antibody

Molecular Identity and Functional Role

YOR114W (now designated CIM1) encodes a mitochondrial HMG-box protein that regulates mtDNA copy number by antagonizing the function of Abf2, another mtDNA-packaging protein . Key features include:

• Structural properties: Contains a high-mobility group (HMG) box domain, enabling DNA binding and protein interactions.

• Functional role:

  • Limits mtDNA copy number via competitive inhibition of Abf2 binding .

  • Participates in mitochondrial genome maintenance under stress conditions .

Table 1: Experimental Insights from CIM1 Studies

Notable Findings:

• Overexpression of CIM1 reduces mtDNA stability, while its deletion increases mtDNA abundance .

• Cim1 interacts with the LON protease system, suggesting a role in protein quality control .

Antibody Applications in Research

The YOR114W antibody is utilized in:

• Western blotting: Confirms protein expression levels in mitochondrial extracts .

• Fluorescence microscopy: Localizes Cim1 within mitochondria using tagged constructs .

• Genetic studies: Validates gene deletion/complementation in strains lacking ABF2 or CIM1 .

Table 2: Common Workflows

Challenges and Considerations

• Cross-reactivity: Validation is required to ensure specificity, given structural similarities among HMG-box proteins .

• Epitope stability: Cim1’s DNA-binding activity may shield epitopes, necessitating optimized extraction protocols .