YPK1 Antibody
Description
Definition and Types
The YPK1 Antibody targets the yeast protein kinase Ypk1, a 99-kDa AGC kinase homologous to human SGK1 . Two primary types of antibodies are described in the literature:
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Pan-specific antibodies: Recognize the full-length Ypk1 protein, suitable for detecting total Ypk1 levels in Western blotting or immunoprecipitation (IP) .
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Phospho-specific antibodies: Detect Ypk1 phosphorylation at specific residues, including the activation loop (A-Loop), hydrophobic motif (HM), and Thr662 . These antibodies are critical for studying Ypk1 activation states under stress conditions.
Applications
YPK1 Antibodies are utilized in diverse experimental approaches:
Role in Plasma Membrane Stress
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Ypk1 localizes to the PM under stress, a process mediated by Slm1/2 proteins. Antibodies demonstrated that Ypk1 fails to localize in slm1Δ slm2Δ mutants .
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Phospho-specific antibodies revealed TORC2-dependent activation of Ypk1 at Thr662, critical for sphingolipid biosynthesis and stress resistance .
Lipid Metabolism
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Ypk1 regulates ceramide synthase activity, increasing sphingolipid production during stress. Antibodies confirmed Ypk1's interaction with ceramide synthase subunits .
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Deletion of YPK1 leads to increased phosphatidylcholine (PC) deacylation, detectable via Western blotting with Ypk1 antibodies .
Pathogenicity in C. neoformans
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Acetylation of Ypk1 at lysines 315/502 modulates its kinase activity. Antibodies (e.g., HA-tagged) demonstrated that deacetylation enhances fungal virulence .
Table 2: Key Research Insights
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Ypk1 activity requires stimulation by TORC2-mediated phosphorylation at Ser644 and Thr662. PMID: 28739659
- Ypk signaling has been identified as a pathway likely activated under conditions of sphingolipid depletion. Potential Ypk1 target proteins have been identified, providing a resource for ongoing mechanistic studies of cellular responses to sphingolipid depletion and the maintenance of sphingolipid homeostasis. PMID: 27717283
- Ypk1 and Plb1 interact to alter sphingolipid metabolism and upregulate PLB1 expression via Crz1. PMID: 25258318
- Research findings expand the scope of cellular processes regulated by Ypk1/2 to include the negative regulation of calcineurin and broaden the repertoire of mechanisms for membrane recruitment and activation of a protein kinase. PMID: 22307609
- Protein kinase Ypk1 phosphorylates regulatory proteins Orm1 and Orm2 to control sphingolipid homeostasis in Saccharomyces cerevisiae. PMID: 22080611
- Data suggest that Ypk1, and possibly the human Sgk1 kinase, affect fatty-acid uptake and thus energy homeostasis through regulating endocytosis. PMID: 20516150
- Research shows that Ypk1 phosphorylates and thereby down-regulates Fpk1. Furthermore, a complex sphingolipid counteracts the down-regulation of Fpk1 by Ypk1. PMID: 19966303
KEGG: sce:YKL126W
STRING: 4932.YKL126W
