YPR146C Antibody
Description
Research Context
The YPR146C protein is implicated in mitochondrial function and autophagy pathways in yeast. Studies using this antibody have focused on:
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Mitochondrial Quality Control: YPR146C is linked to selective mitochondrial degradation via mitophagy, a process critical for cellular homeostasis .
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Protein Localization: The antibody has been used to confirm YPR146C localization in mitochondrial compartments, validating its role in organelle-specific degradation mechanisms .
Validation and Quality Control
Antibody characterization is a critical step in ensuring specificity and reproducibility. The YCharOS initiative—a collaboration between academic and industry partners—emphasizes the use of knockout (KO) cell lines for rigorous validation. For example:
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Western Blotting: KO cell lysates are used to confirm the antibody’s ability to detect YPR146C specifically .
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Immunofluorescence: YCharOS protocols recommend testing antibodies under physiological conditions to assess cross-reactivity .
Such methodologies align with broader antibody validation standards, as outlined in the PLAbDab database, which catalogs over 150,000 antibody sequences and their functional annotations .
Database References
The YPR146C Antibody is likely cataloged in specialized databases:
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
