SPCC18.02 Antibody

Antibody Overview

CCDC18 Antibody (30024-1-AP) is a rabbit-derived polyclonal antibody designed for detecting the human and rat CCDC18 protein, which plays roles in cellular processes such as cytoskeletal organization and ciliary function .

Validated Applications and Performance

This antibody has been rigorously tested across multiple platforms:

Western Blot (WB)

• Detected in: A431 (epidermoid carcinoma), Daudi (Burkitt’s lymphoma), HL-60 (leukemia), and HeLa (cervical cancer) cells .

• Recommended Dilution: 1:1000–1:8000 .

• Key Data: Distinct bands at 151 kDa confirm specificity, though additional bands may arise due to post-translational modifications or splice variants .

Immunohistochemistry (IHC)

• Validated in: Rat colon tissue.

• Antigen Retrieval: TE buffer (pH 9.0) or citrate buffer (pH 6.0) .

• Dilution Range: 1:50–1:500 .

Immunofluorescence (IF/ICC)

• Cellular Localization: Nuclear and cytoplasmic staining observed in U2OS osteosarcoma cells .

Key Functional Attributes

Research Implications

• Disease Studies: CCDC18’s association with ciliary dysfunction makes this antibody valuable for investigating disorders like primary ciliary dyskinesia .

• Technical Considerations:

  • Cross-reactivity with non-target proteins necessitates optimization of blocking buffers (e.g., 5% normal serum) .

  • For live-cell imaging, use Fab fragments to avoid Fc receptor binding artifacts .

Comparative Data from Related Antibodies

While 30024-1-AP targets CCDC18, other antibodies (e.g., ab183039 for SKP2) highlight methodological parallels:

Validation and Quality Control

• Specificity: Loss of signal in SKP2 knockout cells (as demonstrated in analogous antibodies like ab183039) supports target specificity .

• Batch Consistency: Antigen-affinity purification ensures minimal lot-to-lot variability .