YWHAZ (Ab-58) Antibody
Shipped with Ice Packs 
In Stock 
Description
Introduction to YWHAZ (Ab-58) Antibody
The YWHAZ (Ab-58) Antibody is a monoclonal antibody designed to detect phosphorylated YWHAZ at Ser58, a post-translational modification linked to its regulatory functions. This antibody is critical for studying YWHAZ's role in signal transduction, apoptosis, and cancer progression .
YWHAZ (14-3-3ζ/δ) interacts with phosphoserine-containing proteins to modulate pathways such as PI3K/Akt, MAPK, and NF-κB, influencing cell survival, growth, and stress adaptation . The Ab-58 epitope (Ser58) is evolutionarily conserved, making this antibody suitable for cross-species studies in humans, mice, and rats .
Table 2: Cross-Reactivity and Validation
Role in Cancer Biology
-
Bladder Cancer: YWHAZ overexpression correlates with advanced tumor stage, lymph node invasion, and chemotherapy resistance. Knockdown experiments using specific shRNA confirmed its role in suppressing apoptosis .
-
Pancreatic Cancer: A high-throughput screen identified YWHAZ as a metastasis promoter via epithelial-mesenchymal transition (EMT). Overexpression increased metastatic potential in vivo .
Inflammation and Autoimmunity
-
Rheumatoid Arthritis (RA): YWHAZ levels in urine and serum serve as biomarkers for inflammation resolution. Exosomal YWHAZ from regulatory T cells (Tregs) suppresses proinflammatory cytokines, suggesting therapeutic potential .
Validation and Experimental Data
-
Western Blot Protocol:
-
Key Validation Result:
"YWHAZ (Ab-58) Antibody detected phosphorylated YWHAZ in HeLa cells treated with IBMX and forskolin, confirming its responsiveness to cAMP pathway activation ."
Clinical and Therapeutic Implications
-
Cancer Therapeutics: YWHAZ inhibition sensitizes cells to cisplatin, doxorubicin, and radiation .
-
Autoimmune Disease: Adenovirus-mediated YWHAZ delivery reduced arthritis severity in murine models .
Limitations and Future Directions
While the YWHAZ (Ab-58) Antibody is highly specific, its cross-reactivity with YWHAB/H/G isoforms requires careful experimental controls . Future studies should explore its utility in single-cell sequencing and spatial transcriptomics to map phosphorylation dynamics in tumor microenvironments.
Product Specs
Form
Supplied at 1.0 mg/mL in phosphate-buffered saline (without Mg2+ and Ca2+), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Lead Time
Orders are typically dispatched within 1-3 business days of receipt. Delivery times may vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Synonyms
14 3 3 delta antibody; 14 3 3 protein zeta/delta antibody; 14 3 3 protein/cytosolic phospholipase A2 antibody; 14 3 3 zeta antibody; 14-3-3 protein zeta/delta antibody; 1433Z_HUMAN antibody; Epididymis luminal protein 4 antibody; Epididymis secretory protein Li 3 antibody; HEL S 3 antibody; HEL4 antibody; KCIP-1 antibody; KCIP1 antibody; MGC111427 antibody; MGC126532 antibody; MGC138156 antibody; Phospholipase A2 antibody; Protein kinase C inhibitor protein 1 antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; delta polypeptide antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; zeta antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; zeta polypeptide antibody; Tyrosine 3/tryptophan 5 monooxygenase activation protein; zeta polypeptide antibody; YWHAD antibody; YWHAZ antibody
Target Names
Uniprot No.
Target Background
Function
YWHAZ (14-3-3ζ) is an adapter protein that plays a crucial role in regulating a broad range of signaling pathways, both general and specialized. It interacts with numerous binding partners, primarily through recognition of phosphoserine or phosphothreonine motifs. This interaction typically modulates the activity of the binding partner. YWHAZ enhances ARHGEF7 activity on RAC1, promoting lamellipodia and membrane ruffle formation. In neuronal contexts, it regulates spine maturation by modulating ARHGEF7 activity.
Gene References Into Functions
YWHAZ's involvement in various biological processes is supported by extensive research. The following studies highlight its diverse roles and clinical implications:
- Reduced 14-3-3 protein levels observed in the pineal gland, blood platelets, and ileum of individuals with autism spectrum disorder (ASD). PMID: 28522826
- Disruptions in the N-terminal helices of 14-3-3ζ are linked to altered dimer-monomer dynamics and may affect its chaperone function. PMID: 29109150
- YWHAZ knockdown inhibits cell cycle progression and migration, suppresses stem cell marker expression, and reduces tumorigenicity in a mouse model. miR-30e directly downregulates YWHAZ in resistant ovarian cancer cells. PMID: 30134224
- miR-204 and 14-3-3ζ are identified as potential therapeutic targets in osteosarcoma. PMID: 29441884
- Insufficient evidence exists to support 14-3-3ζ as a reliable marker for tamoxifen resistance. PMID: 28643021
- TRIM21 positively regulates osteosarcoma cell proliferation and stress tolerance. YWHAZ is identified as a TRIM21-interacting partner, and its expression is negatively regulated by TRIM21. PMID: 29673441
- 14-3-3 binding protects several disordered regions of PI4KB from proteolytic degradation. PMID: 28864297
- miR-451 inhibits cell migration and invasion, promotes apoptosis, induces cell cycle arrest, and targets YWHAZ. PMID: 28981108
- Elevated serum autoantibodies to YWHAZ are found in gastric cancer patients compared to controls. PMID: 28944820
- A double mutant (L12E, M78K) disrupts 14-3-3ζ dimerization. PMID: 29203375
- YWHAZ is a direct target of miR-613 in hepatocellular carcinoma (HCC), and its overexpression counteracts miR-613's tumor-suppressing effects. PMID: 29551505
- 14-3-3ζ overexpression may serve as a prognostic biomarker for ovarian cancer. PMID: 29214776
- In acute myeloid leukemia (AML), low miR-451 levels correlate with high c-Myc and YWHAZ levels, suggesting a role for the c-Myc-miR-451-YWHAZ/AKT cascade in leukemogenesis. miR-451 restoration may be a therapeutic strategy. PMID: 27764807
- miR-22 exhibits tumor-suppressive effects in hepatocellular carcinoma cells by regulating YWHAZ/AKT/FOXO3a signaling. PMID: 27811373
- 14-3-3ζ overexpression in pre-cancerous breast epithelial cells increases glycolysis and LDHA expression, contributing to breast cancer initiation. PMID: 27150057
- 14-3-3ζ binds to the FOXO3a transcription factor, promoting cytoplasmic export and enhancing proliferation and migration of tongue cancer cells. PMID: 27080223
- The structure of the phosphorylated liver kinase B1 and 14-3-3ζ complex has been reported. PMID: 28368277
- The hypoxia/14-3-3ζ/HIF-1α pathway plays a significant role in portal vein tumor thrombus formation and hepatocellular carcinoma metastasis. PMID: 26910835
- Under high cell density, 14-3-3ζ recruits YAP and p-LATS, forming a complex where 14-3-3ζ acts as a key regulator. PMID: 27334574
- Human procaspase-2 interaction with 14-3-3ζ is regulated by phosphorylation at S139 and S164. PMID: 28943433
- TSC2 protects glioblastoma from photodynamic therapy-induced cell death, and TSC2 and YWHAZ are identified as novel RIP3 partners. PMID: 27984090
- 14-3-3ζ is involved in the TLR3-TICAM-1 pathway, promoting TICAM-1 multimerization and signalosome formation. PMID: 27058640
- Microtubule-bound tau is resistant to 14-3-3ζ-induced aggregation, suggesting that tau phosphorylation promotes aggregation by detaching tau from microtubules. PMID: 27548710
- The structural interface between LRRK2 and 14-3-3δ protein has been characterized. PMID: 28202711
- 14-3-3ζ-mediated cancer cell invasion upregulates Snail via atypical protein kinase C (aPKC) activation. PMID: 27554601
- 14-3-3σ maintains the epithelial phenotype by inhibiting epithelial-to-mesenchymal transition (EMT), potentially contributing to its tumor suppressor function. PMID: 27261462
- 14-3-3ζ regulates HIF-1α production in hepatocellular carcinoma cells by directly binding to HIF-1α and through the PI3K/Akt/NF-κB pathway. PMID: 26884855
- HuR induces 14-3-3ζ translation via interaction with its 3'UTR, and 14-3-3ζ is required for intestinal epithelial cell migration after wounding. PMID: 27401462
- Downregulation of 14-3-3ζ inhibits TGFβ1-induced contraction by decreasing total RhoA expression. PMID: 26906158
- Loss of c-abl expression, but not YWHAZ, is a critical event in tumorigenesis and progression. PMID: 26429164
- The molecular basis for C-Raf C-terminal-derived phosphopeptide interaction with 14-3-3ζ and the structural features mediating phosphorylation-dependent binding have been elucidated. PMID: 26295714
- 14-3-3ζ plays a significant role in breast cancer signaling pathways and positively regulates growth factor receptors and protein kinase pathways. PMID: 25861752
- 14-3-3ζ is overexpressed in oral squamous cell carcinoma and may regulate tumor inflammation and immune response via Stat3 signaling. PMID: 25556369
- PCTAIRE-1 activation is mediated through interaction with phosphorylated cyclin Y in complex with 14-3-3. PMID: 26205494
- The C-terminal domain of Pdc interacts with the 14-3-3 dimer exterior surface. PMID: 25971962
- YWHAZ may be a promising prognostic biomarker in localized prostate cancer, predicting poor prognosis. PMID: 25156059
- Ser9-phosphorylated GSK3β interacts with 14-3-3ζ, and 14-3-3ζ-promoted Ser9-phosphorylation of GSK3β is crucial for NF-κB pathway activation. PMID: 25138042
- The interaction between singly or doubly phosphorylated human tyrosine hydroxylase isoform 1(1-50) peptides and 14-3-3ζ has been analyzed in detail. PMID: 25418103
- BIS targeting induces cellular senescence via regulation of 14-3-3ζ/STAT3/SKP2/p27 in glioblastoma cells. PMID: 25412315
- Aberrant upregulation of 14-3-3σ and EZH2 serves as a poor prognostic biomarker for hepatocellular carcinoma. PMID: 25226601
- 14-3-3ζ-driven changes in Smad partner interactions (from p53 to Gli2) may be biomarkers and therapeutic targets in TGF-β-mediated cancer progression. PMID: 25670079
- YWHAZ and the X-linked DRP2 are potential novel autism candidate genes. PMID: 23999528
- The interaction between 14-3-3ζ and Atg9A is mediated by phosphorylation at Ser761. PMID: 25266655
- miR-375 regulates 14-3-3ζ, affecting telomerase activity by altering TERT nuclear translocation. PMID: 24708873
- 14-3-3ζ regulates PP1α nuclear trafficking in mammalian cells. PMID: 24956593
- Metabolic stress-induced degradation of 14-3-3ζ, by preventing cofilin inactivation, promotes the conversion of blood monocytes into a hypermigratory, proatherogenic phenotype. PMID: 24812321
- Multidrug-resistant HL-60/VCR cells exhibit increased 14-3-3ζ expression compared to HL-60 cells. 14-3-3ζ silencing enhances sensitivity to TPT-induced apoptosis. PMID: 24603438
- 14-3-3ζ causes synaptic loss by destabilizing microtubules and promoting synaptophysin degradation in Alzheimer's disease neurons. PMID: 24367683
- Combined 14-3-3ζ and Hsp27 expression may predict survival in non-small cell lung cancer (NSCLC) and serve as a therapeutic target. PMID: 24804299
- Whole-exome sequencing implicates YWHAZ and PTK2 in castration-resistant prostate cancer. PMID: 24114522
Database Links
Protein Families
14-3-3 family
Subcellular Location
Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
