ZDHHC20 Antibody
Description
Definition and Immunological Profile
The ZDHHC20 antibody is a polyclonal rabbit-derived primary antibody optimized for immunodetection techniques. It specifically targets the ZDHHC20 protein, which mediates S-palmitoylation—a critical modification influencing protein localization, stability, and function . The antibody’s epitope spans amino acids 72–121 of the human ZDHHC20 protein, ensuring specificity for this region .
Key Features:
Applications in Research
The ZDHHC20 antibody is validated for use in:
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Western Blotting: Detects ZDHHC20 in lysates at dilutions of 1:500–1:1000 .
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ELISA: Effective at 1:10,000 dilution for quantifying ZDHHC20 levels .
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Immunohistochemistry (IHC): Demonstrated utility in tissue microarray studies to assess ZDHHC20 expression in cancer samples .
Example Use Cases:
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Cancer Research: ZDHHC20 overexpression correlates with poor prognosis in hepatocellular carcinoma (HCC) and pancreatic ductal adenocarcinoma (PDAC), as shown by IHC and Western blot analyses .
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Viral Pathogenesis: SARS-CoV-2 hijacks ZDHHC20 for Spike protein S-acylation, enhancing viral infectivity .
Cancer Biology
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HCC: ZDHHC20 activates the PI3K-AKT signaling pathway, promoting tumor growth and proliferation . Its knockdown inhibits cancer cell survival and induces apoptosis.
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PDAC: ZDHHC20 stabilizes YTHDF3 via S-palmitoylation, leading to oncogenic MYC accumulation and cancer progression .
Antiviral Mechanisms
ZDHHC20 enhances IFITM3 antiviral activity by palmitoylating lysosomal proteins, restricting influenza virus infection .
SARS-CoV-2 Pathogenesis
ZDHHC20-mediated S-acylation of the Spike protein facilitates viral membrane fusion, a critical step in infection .
Product Specs
Target Background
- The crystal structure of ZDHHC20 has been determined, revealing that its active site resides at the membrane-cytosol interface. The acyl chain binds within a cavity formed by the transmembrane domain. This structural information allows for a proposed mechanism explaining the selectivity for acyl chain length in DHHC enzymes, based on cavity mutants exhibiting preferences for shorter or longer acyl chains. PMID: 29326245
- Targeting ZDHHC20, a peripheral modulator of EGFR signaling, leads to a loss of signal regulation and increased susceptibility to EGFR inhibitor-induced cell death. PMID: 27153536
- ZDHHC20 is a human N-terminal-myristoyl-directed palmitoyl acyltransferase involved in cellular transformation. It may play a role in cancer development. PMID: 20334580
