ZMIZ1 Antibody, FITC conjugated
Description
Antibody Characteristics
| Parameter | Details |
|---|---|
| Target Protein | ZMIZ1 (Human, Mouse, Rat, and others; cross-reactivity varies by source) |
| Epitope | Varies by manufacturer (e.g., C-terminal, N-terminal, or internal regions) |
| Host Species | Rabbit or sheep (polyclonal) |
| Conjugation | FITC (Fluorescein Isothiocyanate) |
| Excitation/Emission | ~495 nm / ~519 nm (fluorescence imaging) |
| Applications | Flow cytometry, immunofluorescence, live-cell tracking, and IHC-Fr |
Key Note: While specific FITC-conjugated ZMIZ1 antibodies are not explicitly listed in the provided sources, standard conjugation practices suggest suppliers like R&D Systems, antibodies-online, and VWR offer FITC-labeled variants tailored for fluorescent detection .
Detection of ZMIZ1 Localization and Activity
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Immunofluorescence (IF): FITC-conjugated ZMIZ1 antibodies enable visualization of nuclear or cytoplasmic ZMIZ1 localization in fixed or live cells. For example, ZMIZ1 is reported to localize to nuclei in human ovary sections and lymphatic endothelial cells (LECs) .
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Flow Cytometry: Quantifies ZMIZ1 expression levels in cell populations. This is critical for studying ZMIZ1’s role in diseases like vitiligo, where its overexpression promotes melanocyte proliferation and migration .
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Live-Cell Imaging: FITC’s photostability allows real-time tracking of ZMIZ1 dynamics during processes like lymphatic valve formation .
Mechanistic Studies in Disease Models
ZMIZ1 in Melanocyte Biology
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Vitiligo Pathogenesis: Overexpression of ZMIZ1 in melanocyte cell lines (e.g., PIG1, PIG3V) increases proliferation, reduces apoptosis, and enhances migration. FITC-conjugated antibodies could track ZMIZ1’s subcellular distribution during these processes .
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Cytoskeletal Remodeling: ZMIZ1 knockdown alters actin cytoskeleton structure, impairing cell migration. Fluorescent imaging with FITC-labeled antibodies could visualize cytoskeletal dynamics .
ZMIZ1 in Cancer
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TSCC Progression: ZMIZ1 knockdown reduces Notch1/Jagged1 signaling and metastasis-related factors (MMP7, MKP-1). FITC-conjugated antibodies may aid in assessing ZMIZ1’s interaction with Notch1 in real-time .
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Lymphatic Endothelial Cells (LECs): ZMIZ1 regulates Prox1 expression by modifying chromatin accessibility at its regulatory regions. FITC-labeled antibodies could map ZMIZ1’s recruitment to Prox1 enhancers during lymphangiogenesis .
ZMIZ1 in Vascular Development
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Yolk Sac Vasculature: Global ZMIZ1 knockout mice exhibit embryonic lethality due to defective yolk sac vascularization. FITC-conjugated antibodies may trace ZMIZ1’s role in endothelial cell survival or migration .
Comparison of ZMIZ1 Antibodies
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- A genome-wide association study and subsequent replication study identified a significant (P<5 x 10(-8)) association between rs76518691 in the ZMIZ1 gene and rs7523831 near NGF, and primary dysmenorrhea in Chinese women. PMID: 28447608
- Research has identified a molecular phenotype of Multiple Sclerosis (MS) characterized by the expression of the MS risk gene ZMIZ1 in blood, along with other genes, particularly transcription factors. Notably, ZMIZ1 expression is influenced by and interacts with environmental risk factors such as Epstein-Barr virus (EBV) and Vitamin D. PMID: 28063629
- The variants rs1250569 (ZMIZ1) and rs10114470 (TL1A) have been identified as novel loci associated with susceptibility to Inflammatory Bowel Disease in Han-Chinese patients. PMID: 28456797
- This case provides valuable insights into the potential function and effects of MIZ-type containing and proline-rich 12 in the central nervous system, as it represents the first constitutional balanced translocation disrupting and fusing these two elements. PMID: 26163108
- Research at the ZMIZ1 locus has revealed that perturbation of ZMIZ1 expression in human islets and beta-cells impacts exocytosis and insulin secretion, highlighting a novel role for ZMIZ1 in maintaining glucose homeostasis. PMID: 26624892
- Targeting the interaction between NOTCH1 and ZMIZ1 could potentially be a strategy for combating leukemic growth. PMID: 26522984
- The expression of SENP8, SAE1, PIAS1, PIAS2, and ZMIZ1 is dysregulated in the majority of papillary thyroid carcinoma (PTC) tissues, suggesting a potential contribution to the PTC phenotype. PMID: 26403403
- ZMIZ1 has been identified as a susceptibility gene for vitiligo in the Chinese population. PMID: 24667117
- ZMIZ1 is overexpressed in a significant proportion of human breast, ovarian, and colon cancers, as well as squamous cell carcinomas, suggesting a broader role in epithelial cancers. PMID: 23426136
- ZMIZ1 and activated NOTCH1 are coexpressed in a subset of human T-cell acute lymphoblastic leukemia (T-ALL) patients and cell lines. PMID: 23161489
- Evidence suggests a physiological role for endogenous Zimp10 in regulating Smad3/4-mediated transcription. PMID: 16777850
- RAI17 was found to be upregulated in WT-Add1 compared to MUT-Add1 overexpressing cells, potentially representing a key molecule/axis for the functional Add1-induced effect. PMID: 17512505
- Expression of exogenous hZimp10 enhances the transcriptional activity of p53, while knockdown of endogenous hZimp10 reduces the transcriptional activity of p53. PMID: 17584785
- Research provides evidence supporting a crucial role for Zimp10 in vasculogenesis. PMID: 17967885
- Fusion of ZMIZ1 to ABL1 is associated with a B-cell acute lymphoblastic leukemia with a t(9;10)(q34;q22.3) translocation. PMID: 18007576
